@inbook{cfca7aee24df4142a255238395a2521c,
title = "Xenopus transgenics: Methods using transposons",
abstract = "The generation of transgenic animals is an essential tool for many genetic strategies. DNA cut-and-paste transposon systems can be used to efficiently modify the Xenopus genome. The DNA transposon substrate, harbored on a circularized plasmid, is co-injected into fertilized Xenopus embryos at the one-cell stage together with mRNA encoding the cognate transposase enzyme. The cellular machinery rapidly translates the exogenous mRNA to produce active transposase enzyme that catalyzes excision of the transposon substrate from the plasmid and stable integration into the genomic DNA.",
keywords = "Sleeping beauty, Tol2, Transgenesis, Transposon, Xenopus",
author = "Kelley, \{Clair M.\} and Yergeau, \{Donald A.\} and Haiqing Zhu and Emin Kuliyev and Mead, \{Paul E.\}",
year = "2012",
doi = "10.1007/978-1-61779-992-1\_14",
language = "English",
isbn = "9781617799914",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "231--243",
booktitle = "Xenopus Protocols",
}