X-ray crystallographic analysis of the hydration of A- and B-form DNA at atomic resolution

Martin Egli; Valentine Tereshko; Marianna Teplova; George Minasov; Andrzei Joachimiak; Ruslan Sanishvili; Charles M. Weeks; Russ Miller; Martin A. Maier; Haoyun An; P. Dan Cook; Muthiah Manoharan

doi:10.1002/(SICI)1097-0282(1998)48:4<234::AID-BIP4>3.0.CO;2-H

X-ray crystallographic analysis of the hydration of A- and B-form DNA at atomic resolution

Martin Egli
, Valentine Tereshko
, Marianna Teplova
, George Minasov
, Andrzei Joachimiak
, Ruslan Sanishvili
, Charles M. Weeks
, Russ Miller
, Martin A. Maier
, Haoyun An
, P. Dan Cook
, Muthiah Manoharan

Department of Computer Science and Engineering

Northwestern University
Argonne National Laboratory
Hauptman-Woodward Medical Research Institute, Inc.
SUNY Buffalo
Ionis Pharmaceuticals

Research output: Contribution to journal › Article › peer-review

147 Scopus citations

Abstract

We have determined single crystal structures of an A-DNA decanter and a B-DNA dodecamer at 0.83 and 0.95 A, respectively. The resolution of the fanner is the highest reported thus far for any right-handed nucleic acid duplex and the quality of the diffraction data allowed determination of the structure with direct methods. The structures reveal unprecedented details of DNA fine structure and hydration; in particular, we have reexamined the overall hydration of A- and B-fonn DNA, the distribution of water around phosphate groups, and features of the water structure that may underlie the B to A transition.

Original language	English
Pages (from-to)	234-252
Number of pages	19
Journal	Biopolymers
Volume	48
Issue number	4
DOIs	https://doi.org/10.1002/(SICI)1097-0282(1998)48:4<234::AID-BIP4>3.0.CO;2-H
State	Published - 1998

Keywords

A-DNA
B-DNA
Direct methods
Hydration
Structural transition
Water structure
X-ray crystallography

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10.1002/(SICI)1097-0282(1998)48:4<234::AID-BIP4>3.0.CO;2-H

Cite this

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title = "X-ray crystallographic analysis of the hydration of A- and B-form DNA at atomic resolution",

abstract = "We have determined single crystal structures of an A-DNA decanter and a B-DNA dodecamer at 0.83 and 0.95 A, respectively. The resolution of the fanner is the highest reported thus far for any right-handed nucleic acid duplex and the quality of the diffraction data allowed determination of the structure with direct methods. The structures reveal unprecedented details of DNA fine structure and hydration; in particular, we have reexamined the overall hydration of A- and B-fonn DNA, the distribution of water around phosphate groups, and features of the water structure that may underlie the B to A transition.",

keywords = "A-DNA, B-DNA, Direct methods, Hydration, Structural transition, Water structure, X-ray crystallography",

author = "Martin Egli and Valentine Tereshko and Marianna Teplova and George Minasov and Andrzei Joachimiak and Ruslan Sanishvili and Weeks, \{Charles M.\} and Russ Miller and Maier, \{Martin A.\} and Haoyun An and \{Dan Cook\}, P. and Muthiah Manoharan",

year = "1998",

doi = "10.1002/(SICI)1097-0282(1998)48:4<234::AID-BIP4>3.0.CO;2-H",

language = "English",

volume = "48",

pages = "234--252",

journal = "Biopolymers",

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publisher = "John Wiley and Sons Inc",

number = "4",

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TY - JOUR

T1 - X-ray crystallographic analysis of the hydration of A- and B-form DNA at atomic resolution

AU - Egli, Martin

AU - Tereshko, Valentine

AU - Teplova, Marianna

AU - Minasov, George

AU - Joachimiak, Andrzei

AU - Sanishvili, Ruslan

AU - Weeks, Charles M.

AU - Miller, Russ

AU - Maier, Martin A.

AU - An, Haoyun

AU - Dan Cook, P.

AU - Manoharan, Muthiah

PY - 1998

Y1 - 1998

N2 - We have determined single crystal structures of an A-DNA decanter and a B-DNA dodecamer at 0.83 and 0.95 A, respectively. The resolution of the fanner is the highest reported thus far for any right-handed nucleic acid duplex and the quality of the diffraction data allowed determination of the structure with direct methods. The structures reveal unprecedented details of DNA fine structure and hydration; in particular, we have reexamined the overall hydration of A- and B-fonn DNA, the distribution of water around phosphate groups, and features of the water structure that may underlie the B to A transition.

AB - We have determined single crystal structures of an A-DNA decanter and a B-DNA dodecamer at 0.83 and 0.95 A, respectively. The resolution of the fanner is the highest reported thus far for any right-handed nucleic acid duplex and the quality of the diffraction data allowed determination of the structure with direct methods. The structures reveal unprecedented details of DNA fine structure and hydration; in particular, we have reexamined the overall hydration of A- and B-fonn DNA, the distribution of water around phosphate groups, and features of the water structure that may underlie the B to A transition.

KW - A-DNA

KW - B-DNA

KW - Direct methods

KW - Hydration

KW - Structural transition

KW - Water structure

KW - X-ray crystallography

UR - https://www.scopus.com/pages/publications/0000742485

U2 - 10.1002/(SICI)1097-0282(1998)48:4<234::AID-BIP4>3.0.CO;2-H

DO - 10.1002/(SICI)1097-0282(1998)48:4<234::AID-BIP4>3.0.CO;2-H

M3 - Article

C2 - 10699842

AN - SCOPUS:0000742485

SN - 0006-3525

VL - 48

SP - 234

EP - 252

JO - Biopolymers

JF - Biopolymers

IS - 4

ER -

X-ray crystallographic analysis of the hydration of A- and B-form DNA at atomic resolution

Abstract

Keywords

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