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Wavelength dependent damage in biological multi-photon microscopy: Ti:Sapphire vs. Cr:Forsterite lasers

  • C. K. Sun
  • , I. H. Chen
  • , S. W. Chu
  • , B. L. Lin
  • , P. C. Cheng
  • National Taiwan University

Research output: Contribution to journalConference articlepeer-review

Abstract

Wavelength dependent on-focus cell damage in multi-photon confocal microscopy with femtosecond infrared laser sources was studied. Experiments performed in selected plant tissues showed the occurrence of multi-photon absorption induced cell damages due to plasma generations and strong luminescence. The dosages required to produce plasma generation were found to decrease with increase illumination intensity. Sustained multi-photon spectra were observed in most plant specimens with a tightly focused Cr:forsterite laser beam under long term irradiation.

Original languageEnglish
Pages (from-to)393-394
Number of pages2
JournalConference Proceedings - Lasers and Electro-Optics Society Annual Meeting-LEOS
Volume1
StatePublished - 2001
Event14th Annual Meeting of the IEEE Lasers and Electro-Optics Society - San Diego, CA, United States
Duration: Nov 11 2001Nov 15 2001

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