Vyiavlenie tkanespetsificheskoǐ ékspressii genov metodom gibridizatsii in situ na otpechatkakh gistologicheskikh srezov.

K. N. Kashkin; S. M. Troianovskiǐ; A. V. Gudkov

Vyiavlenie tkanespetsificheskoǐ ékspressii genov metodom gibridizatsii in situ na otpechatkakh gistologicheskikh srezov.

Translated title of the contribution: Detection of tissue-specific gene expression using an in situ hybridization method of histoblotting

K. N. Kashkin
, S. M. Troianovskiǐ
, A. V. Gudkov

Roswell Park - Cell Stress and Biophysical Oncology

Research output: Contribution to journal › Article › peer-review

Abstract

A version of in situ hybridization on the histological sections that is used for screening specific mRNA in tissues in proposed. Sections of the frozen tissue samples are prepared on the cryostatic microtome, placed on nitrocellulose filters and hybridized with labelled DNA-probes under the conditions of RNA blot hybridization. The proposed method ("histoblotting") was used to study the distribution of actin and alpha-phetoprotein mRNA genes in tissues of 15 and 21-day rat embryos. The possibility of studying mRNA (by hibridizations) and protein (by immunoenzyme staining) and making histological analysis simultaneously by histoblotting is demonstrated.

Translated title of the contribution	Detection of tissue-specific gene expression using an in situ hybridization method of histoblotting
Original language	Russian
Pages (from-to)	16-18
Number of pages	3
Journal	Molekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number	9
State	Published - Sep 1987

Cite this

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title = "Vyiavlenie tkanespetsificheskoǐ {\'e}kspressii genov metodom gibridizatsii in situ na otpechatkakh gistologicheskikh srezov.",

abstract = "A version of in situ hybridization on the histological sections that is used for screening specific mRNA in tissues in proposed. Sections of the frozen tissue samples are prepared on the cryostatic microtome, placed on nitrocellulose filters and hybridized with labelled DNA-probes under the conditions of RNA blot hybridization. The proposed method ({"}histoblotting{"}) was used to study the distribution of actin and alpha-phetoprotein mRNA genes in tissues of 15 and 21-day rat embryos. The possibility of studying mRNA (by hibridizations) and protein (by immunoenzyme staining) and making histological analysis simultaneously by histoblotting is demonstrated.",

author = "Kashkin, \{K. N.\} and Troianovskiǐ, \{S. M.\} and Gudkov, \{A. V.\}",

year = "1987",

month = sep,

language = "Russian",

pages = "16--18",

journal = "Molekuliarnaia genetika, mikrobiologiia i virusologiia",

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T1 - Vyiavlenie tkanespetsificheskoǐ ékspressii genov metodom gibridizatsii in situ na otpechatkakh gistologicheskikh srezov.

AU - Kashkin, K. N.

AU - Troianovskiǐ, S. M.

AU - Gudkov, A. V.

PY - 1987/9

Y1 - 1987/9

N2 - A version of in situ hybridization on the histological sections that is used for screening specific mRNA in tissues in proposed. Sections of the frozen tissue samples are prepared on the cryostatic microtome, placed on nitrocellulose filters and hybridized with labelled DNA-probes under the conditions of RNA blot hybridization. The proposed method ("histoblotting") was used to study the distribution of actin and alpha-phetoprotein mRNA genes in tissues of 15 and 21-day rat embryos. The possibility of studying mRNA (by hibridizations) and protein (by immunoenzyme staining) and making histological analysis simultaneously by histoblotting is demonstrated.

AB - A version of in situ hybridization on the histological sections that is used for screening specific mRNA in tissues in proposed. Sections of the frozen tissue samples are prepared on the cryostatic microtome, placed on nitrocellulose filters and hybridized with labelled DNA-probes under the conditions of RNA blot hybridization. The proposed method ("histoblotting") was used to study the distribution of actin and alpha-phetoprotein mRNA genes in tissues of 15 and 21-day rat embryos. The possibility of studying mRNA (by hibridizations) and protein (by immunoenzyme staining) and making histological analysis simultaneously by histoblotting is demonstrated.

UR - https://www.scopus.com/pages/publications/0023403034

M3 - Article

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AN - SCOPUS:0023403034

SN - 0208-0613

SP - 16

EP - 18

JO - Molekuliarnaia genetika, mikrobiologiia i virusologiia

JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia

IS - 9

ER -

Vyiavlenie tkanespetsificheskoǐ ékspressii genov metodom gibridizatsii in situ na otpechatkakh gistologicheskikh srezov.

Abstract

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