Vitronectin-dependent invasion of epithelial cells by Neisseria gonorrhoeae involves α(v) integrin receptors

Michaela Dehio; Oscar G. Gómez-Duarte; Christoph Dehio; Thomas F. Meyer

doi:10.1016/S0014-5793(98)00144-6

Vitronectin-dependent invasion of epithelial cells by Neisseria gonorrhoeae involves α(v) integrin receptors

Michaela Dehio
, Oscar G. Gómez-Duarte
, Christoph Dehio
, Thomas F. Meyer

Department of Epidemiology and Environmental Health

Administrative Headquarters of the Max Planck Society
Max Planck Institute for Infection Biology

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa₅₀ protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-triggered bacterial uptake. Blocking integrin function by RGDS peptides or by antibodies specific to α(v)β₅ or α(v)β₃ resulted in an abrogation of VN-triggered bacterial internalization. Moreover, inhibitors of PKC were found to block VN-triggered uptake. The essential role of α(v) integrins and the presumable involvement of PKC in VN-triggered gonococcal uptake are discussed.

Original language	English
Pages (from-to)	84-88
Number of pages	5
Journal	FEBS Letters
Volume	424
Issue number	1-2
DOIs	https://doi.org/10.1016/S0014-5793(98)00144-6
State	Published - Mar 6 1998

Keywords

Integrin
Invasion
Neisseria gonorrhoeae
Protein kinase C
Vitronectin

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10.1016/S0014-5793(98)00144-6

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title = "Vitronectin-dependent invasion of epithelial cells by Neisseria gonorrhoeae involves α(v) integrin receptors",

abstract = "Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-triggered bacterial uptake. Blocking integrin function by RGDS peptides or by antibodies specific to α(v)β5 or α(v)β3 resulted in an abrogation of VN-triggered bacterial internalization. Moreover, inhibitors of PKC were found to block VN-triggered uptake. The essential role of α(v) integrins and the presumable involvement of PKC in VN-triggered gonococcal uptake are discussed.",

keywords = "Integrin, Invasion, Neisseria gonorrhoeae, Protein kinase C, Vitronectin",

author = "Michaela Dehio and G{\'o}mez-Duarte, \{Oscar G.\} and Christoph Dehio and Meyer, \{Thomas F.\}",

year = "1998",

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doi = "10.1016/S0014-5793(98)00144-6",

language = "English",

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journal = "FEBS Letters",

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TY - JOUR

T1 - Vitronectin-dependent invasion of epithelial cells by Neisseria gonorrhoeae involves α(v) integrin receptors

AU - Dehio, Michaela

AU - Gómez-Duarte, Oscar G.

AU - Dehio, Christoph

AU - Meyer, Thomas F.

PY - 1998/3/6

Y1 - 1998/3/6

N2 - Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-triggered bacterial uptake. Blocking integrin function by RGDS peptides or by antibodies specific to α(v)β5 or α(v)β3 resulted in an abrogation of VN-triggered bacterial internalization. Moreover, inhibitors of PKC were found to block VN-triggered uptake. The essential role of α(v) integrins and the presumable involvement of PKC in VN-triggered gonococcal uptake are discussed.

AB - Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-triggered bacterial uptake. Blocking integrin function by RGDS peptides or by antibodies specific to α(v)β5 or α(v)β3 resulted in an abrogation of VN-triggered bacterial internalization. Moreover, inhibitors of PKC were found to block VN-triggered uptake. The essential role of α(v) integrins and the presumable involvement of PKC in VN-triggered gonococcal uptake are discussed.

KW - Integrin

KW - Invasion

KW - Neisseria gonorrhoeae

KW - Protein kinase C

KW - Vitronectin

UR - https://www.scopus.com/pages/publications/0032489349

U2 - 10.1016/S0014-5793(98)00144-6

DO - 10.1016/S0014-5793(98)00144-6

M3 - Article

C2 - 9537520

AN - SCOPUS:0032489349

SN - 0014-5793

VL - 424

SP - 84

EP - 88

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Vitronectin-dependent invasion of epithelial cells by Neisseria gonorrhoeae involves α(v) integrin receptors

Abstract

Keywords

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