Use of a replica‐plate assay for the rapid assessment of salivary protein‐bacteria interactions

Ching‐Chung ‐C Tseng; Frank A. Scannapieco; Michael J. Levine

doi:10.1111/j.1399-302X.1992.tb00021.x

Use of a replica‐plate assay for the rapid assessment of salivary protein‐bacteria interactions

Ching‐Chung ‐C Tseng
, Frank A. Scannapieco
, Michael J. Levine

Department of Oral Biology

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A replica‐plate assay was used to screen for the interaction of salivary molecules with dental plaque bacteria. Bacterial colonies cultured from supragingival plaque on sheep‐blood (SB) agar were replica‐plated onto nitrocellulose membranes overlaying SB or mitis‐salivarius agar. Membranes with attached colonies were removed and incubated with ¹²⁵I‐amylase or ¹²⁵I ‐proline‐rich glycoprotein (PRG). Positive interactions were detected by autoradiography. Only strains of Streptococcus gordonii and Actinomyces viscosus bound amylase, and strains of A. viscosus bound PRG. The results suggest that amylase and PRG bind to selected species of aerobic dental plaque bacteria.

Original language	English
Pages (from-to)	53-56
Number of pages	4
Journal	Oral Microbiology and Immunology
Volume	7
Issue number	1
DOIs	https://doi.org/10.1111/j.1399-302X.1992.tb00021.x
State	Published - Feb 1992

Keywords

Actinomyces viscosus
amylase
dental plaque
praline‐rich glycoprotein
saliva
Streptococcus gordonii

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10.1111/j.1399-302X.1992.tb00021.x

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title = "Use of a replica‐plate assay for the rapid assessment of salivary protein‐bacteria interactions",

abstract = "A replica‐plate assay was used to screen for the interaction of salivary molecules with dental plaque bacteria. Bacterial colonies cultured from supragingival plaque on sheep‐blood (SB) agar were replica‐plated onto nitrocellulose membranes overlaying SB or mitis‐salivarius agar. Membranes with attached colonies were removed and incubated with 125I‐amylase or 125I ‐proline‐rich glycoprotein (PRG). Positive interactions were detected by autoradiography. Only strains of Streptococcus gordonii and Actinomyces viscosus bound amylase, and strains of A. viscosus bound PRG. The results suggest that amylase and PRG bind to selected species of aerobic dental plaque bacteria.",

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AU - Tseng, Ching‐Chung ‐C

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AU - Levine, Michael J.

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AB - A replica‐plate assay was used to screen for the interaction of salivary molecules with dental plaque bacteria. Bacterial colonies cultured from supragingival plaque on sheep‐blood (SB) agar were replica‐plated onto nitrocellulose membranes overlaying SB or mitis‐salivarius agar. Membranes with attached colonies were removed and incubated with 125I‐amylase or 125I ‐proline‐rich glycoprotein (PRG). Positive interactions were detected by autoradiography. Only strains of Streptococcus gordonii and Actinomyces viscosus bound amylase, and strains of A. viscosus bound PRG. The results suggest that amylase and PRG bind to selected species of aerobic dental plaque bacteria.

KW - Actinomyces viscosus

KW - amylase

KW - dental plaque

KW - praline‐rich glycoprotein

KW - saliva

KW - Streptococcus gordonii

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JO - Oral Microbiology and Immunology

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Use of a replica‐plate assay for the rapid assessment of salivary protein‐bacteria interactions

Abstract

Keywords

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