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Use of a cDNA Array for the Identification of Genes Induced in Islets of Suckling Rats by a High-Carbohydrate Nutritional Intervention

  • SUNY Buffalo

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Four-day-old rat pups that are raised artificially on a high-carbohydrate (HC) milk formula immediately develop hyperinsulinemia, which persists into adulthood without any further nutritional stimulus. cDNA array analysis was used to identify large-scale changes in gene expression patterns in islets from 12- and 100-day-old HC rats in response to the HC dietary modification during the suckling period. It was observed that the expression of several genes that belong to clusters involved in β-cell development and/or β-cell function was significantly upregulated in islets from 12- and 100-day-old HC rats. It is inferred that in addition to predicted changes in gene expression, for example pre-proinsulin gene, global changes in gene expression contribute to the hyperinsulinemic state in the HC rat.

Original languageEnglish
Pages (from-to)2053-2060
Number of pages8
JournalDiabetes
Volume50
Issue number9
DOIs
StatePublished - Sep 2001

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