Urotensin II lowers cytoplasmic free calcium concentration in goby enterocytes: Measurements using quin2

Christopher A. Loretz; John A. Assad

doi:10.1016/0016-6480(86)90068-7

Urotensin II lowers cytoplasmic free calcium concentration in goby enterocytes: Measurements using quin2

Christopher A. Loretz
, John A. Assad

Biological Sciences

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A technique is described for the measurement of cytoplasmic free calcium concentration ([Ca²⁺]_c) in a suspension of goby intestinal epithelial cells using the fluorescent probe quin2. For 18 cell suspensions, [Ca²⁺]_c was determined to be 142 ± 14 nM. The caudal neurosecretory peptide urotensin II at 10^-7 to 10^-6 M concentration reduced [Ca²⁺]_c by 20-50%. Together with previous findings linking the calcium-calmodulin system with the stimulation of Na⁺ and Cl^- absorption in this tissue, it appears that alterations in cytoplasmic Ca²⁺ may mediate urotensin II-induced stimulation of ion transport.

Original language	English
Pages (from-to)	355-361
Number of pages	7
Journal	General and Comparative Endocrinology
Volume	64
Issue number	3
DOIs	https://doi.org/10.1016/0016-6480(86)90068-7
State	Published - Dec 1986

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title = "Urotensin II lowers cytoplasmic free calcium concentration in goby enterocytes: Measurements using quin2",

abstract = "A technique is described for the measurement of cytoplasmic free calcium concentration ([Ca2+]c) in a suspension of goby intestinal epithelial cells using the fluorescent probe quin2. For 18 cell suspensions, [Ca2+]c was determined to be 142 ± 14 nM. The caudal neurosecretory peptide urotensin II at 10-7 to 10-6 M concentration reduced [Ca2+]c by 20-50\%. Together with previous findings linking the calcium-calmodulin system with the stimulation of Na+ and Cl- absorption in this tissue, it appears that alterations in cytoplasmic Ca2+ may mediate urotensin II-induced stimulation of ion transport.",

author = "Loretz, \{Christopher A.\} and Assad, \{John A.\}",

year = "1986",

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doi = "10.1016/0016-6480(86)90068-7",

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T1 - Urotensin II lowers cytoplasmic free calcium concentration in goby enterocytes

T2 - Measurements using quin2

AU - Loretz, Christopher A.

AU - Assad, John A.

PY - 1986/12

Y1 - 1986/12

N2 - A technique is described for the measurement of cytoplasmic free calcium concentration ([Ca2+]c) in a suspension of goby intestinal epithelial cells using the fluorescent probe quin2. For 18 cell suspensions, [Ca2+]c was determined to be 142 ± 14 nM. The caudal neurosecretory peptide urotensin II at 10-7 to 10-6 M concentration reduced [Ca2+]c by 20-50%. Together with previous findings linking the calcium-calmodulin system with the stimulation of Na+ and Cl- absorption in this tissue, it appears that alterations in cytoplasmic Ca2+ may mediate urotensin II-induced stimulation of ion transport.

AB - A technique is described for the measurement of cytoplasmic free calcium concentration ([Ca2+]c) in a suspension of goby intestinal epithelial cells using the fluorescent probe quin2. For 18 cell suspensions, [Ca2+]c was determined to be 142 ± 14 nM. The caudal neurosecretory peptide urotensin II at 10-7 to 10-6 M concentration reduced [Ca2+]c by 20-50%. Together with previous findings linking the calcium-calmodulin system with the stimulation of Na+ and Cl- absorption in this tissue, it appears that alterations in cytoplasmic Ca2+ may mediate urotensin II-induced stimulation of ion transport.

UR - https://www.scopus.com/pages/publications/0022998208

U2 - 10.1016/0016-6480(86)90068-7

DO - 10.1016/0016-6480(86)90068-7

M3 - Article

C2 - 3803890

AN - SCOPUS:0022998208

SN - 0016-6480

VL - 64

SP - 355

EP - 361

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

IS - 3

ER -

Urotensin II lowers cytoplasmic free calcium concentration in goby enterocytes: Measurements using quin2

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