Ultra-sensitive immunoassays using multi-photon-detection in diagnostic proteomics of blood

Andrzej K. Drukier; Natascha Ossetrova; Elena Schors; Larry R. Brown; John Tomaszewski; Richard Sainsbury; Jasminka Godovac-Zimmermann

doi:10.1021/pr050320n

Ultra-sensitive immunoassays using multi-photon-detection in diagnostic proteomics of blood

Andrzej K. Drukier
, Natascha Ossetrova
, Elena Schors
, Larry R. Brown
, John Tomaszewski
, Richard Sainsbury
, Jasminka Godovac-Zimmermann

Department of Pathology and Anatomical Sciences

BioTraces Inc.
University College London

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Multiphoton-detection methods that detect as little as 1000 atoms of ¹²⁵I-streptavidin increase the sensitivity of immunoassays by 200- to 1000-fold (1 femtogram/mL). Improved background suppression allows 20- to 100-fold improvements in sensitivity for conventional immunoassays (10-50 femtogram/mL). Quantitation of low abundance biomarkers in blood (PSA, TNFα, VEGF, IL-1β, IL-6, and IL-8), for the first time for complete patient cohorts, indicates that very high analytical sensitivity and new statistical methods are crucial for serum-based diagnostic proteomics.

Original language	English
Pages (from-to)	2375-2378
Number of pages	4
Journal	Journal of Proteome Research
Volume	4
Issue number	6
DOIs	https://doi.org/10.1021/pr050320n
State	Published - Nov 2005

Keywords

Blood
Breast cancer
Cytokines
Diagnostics
Growth factors
HIV-1
Immunoassay
Multiphoton-detection
Proteomics
Serum

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10.1021/pr050320n

Cite this

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title = "Ultra-sensitive immunoassays using multi-photon-detection in diagnostic proteomics of blood",

abstract = "Multiphoton-detection methods that detect as little as 1000 atoms of 125I-streptavidin increase the sensitivity of immunoassays by 200- to 1000-fold (1 femtogram/mL). Improved background suppression allows 20- to 100-fold improvements in sensitivity for conventional immunoassays (10-50 femtogram/mL). Quantitation of low abundance biomarkers in blood (PSA, TNFα, VEGF, IL-1β, IL-6, and IL-8), for the first time for complete patient cohorts, indicates that very high analytical sensitivity and new statistical methods are crucial for serum-based diagnostic proteomics.",

keywords = "Blood, Breast cancer, Cytokines, Diagnostics, Growth factors, HIV-1, Immunoassay, Multiphoton-detection, Proteomics, Serum",

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year = "2005",

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doi = "10.1021/pr050320n",

language = "English",

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T1 - Ultra-sensitive immunoassays using multi-photon-detection in diagnostic proteomics of blood

AU - Drukier, Andrzej K.

AU - Ossetrova, Natascha

AU - Schors, Elena

AU - Brown, Larry R.

AU - Tomaszewski, John

AU - Sainsbury, Richard

AU - Godovac-Zimmermann, Jasminka

PY - 2005/11

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AB - Multiphoton-detection methods that detect as little as 1000 atoms of 125I-streptavidin increase the sensitivity of immunoassays by 200- to 1000-fold (1 femtogram/mL). Improved background suppression allows 20- to 100-fold improvements in sensitivity for conventional immunoassays (10-50 femtogram/mL). Quantitation of low abundance biomarkers in blood (PSA, TNFα, VEGF, IL-1β, IL-6, and IL-8), for the first time for complete patient cohorts, indicates that very high analytical sensitivity and new statistical methods are crucial for serum-based diagnostic proteomics.

KW - Blood

KW - Breast cancer

KW - Cytokines

KW - Diagnostics

KW - Growth factors

KW - HIV-1

KW - Immunoassay

KW - Multiphoton-detection

KW - Proteomics

KW - Serum

UR - https://www.scopus.com/pages/publications/29144460192

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M3 - Article

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AN - SCOPUS:29144460192

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EP - 2378

JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 6

ER -

Ultra-sensitive immunoassays using multi-photon-detection in diagnostic proteomics of blood

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Keywords

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