Two-photon laser scanning fluorescence microscopy - From a fluorophore and specimen perspective

J. D. Bhawalkar; A. Shih; S. J. Pan; W. S. Liou; J. Swiatkiewicz; B. A. Reinhardt; P. N. Prasad; P. C. Cheng

doi:10.1002/1361-6374(199609)4:3<168::aid-bio7>3.0.co;2-8

Two-photon laser scanning fluorescence microscopy - From a fluorophore and specimen perspective

J. D. Bhawalkar
, A. Shih
, S. J. Pan
, W. S. Liou
, J. Swiatkiewicz
, B. A. Reinhardt
, P. N. Prasad
, P. C. Cheng

SUNY Buffalo
Air Force Research Laboratory

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Two-photon laser scanning microscopy is discussed from a fluorophore and specimen point of view. Issues to be considered in the selection of the fluorophore and in specimen preparation for multiphoton microscopy are described. The transverse resolution is estimated, based on a photobleaching method. We also show that the performance of a multiphoton laser scanning microscope is significantly enhanced by the use of a confocal aperture.

Original language	English
Pages (from-to)	168-178
Number of pages	11
Journal	Bioimaging
Volume	4
Issue number	3
DOIs	https://doi.org/10.1002/1361-6374(199609)4:3<168::aid-bio7>3.0.co;2-8
State	Published - 1996

Keywords

confocal microscopy
fluorescence microscopy
two-photon

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10.1002/1361-6374(199609)4:3<168::aid-bio7>3.0.co;2-8

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title = "Two-photon laser scanning fluorescence microscopy - From a fluorophore and specimen perspective",

abstract = "Two-photon laser scanning microscopy is discussed from a fluorophore and specimen point of view. Issues to be considered in the selection of the fluorophore and in specimen preparation for multiphoton microscopy are described. The transverse resolution is estimated, based on a photobleaching method. We also show that the performance of a multiphoton laser scanning microscope is significantly enhanced by the use of a confocal aperture.",

keywords = "confocal microscopy, fluorescence microscopy, two-photon",

author = "Bhawalkar, \{J. D.\} and A. Shih and Pan, \{S. J.\} and Liou, \{W. S.\} and J. Swiatkiewicz and Reinhardt, \{B. A.\} and Prasad, \{P. N.\} and Cheng, \{P. C.\}",

year = "1996",

doi = "10.1002/1361-6374(199609)4:3<168::aid-bio7>3.0.co;2-8",

language = "English",

volume = "4",

pages = "168--178",

journal = "Bioimaging",

issn = "0966-9051",

publisher = "John Wiley \& Sons Inc.",

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TY - JOUR

T1 - Two-photon laser scanning fluorescence microscopy - From a fluorophore and specimen perspective

AU - Bhawalkar, J. D.

AU - Shih, A.

AU - Pan, S. J.

AU - Liou, W. S.

AU - Swiatkiewicz, J.

AU - Reinhardt, B. A.

AU - Prasad, P. N.

AU - Cheng, P. C.

PY - 1996

Y1 - 1996

N2 - Two-photon laser scanning microscopy is discussed from a fluorophore and specimen point of view. Issues to be considered in the selection of the fluorophore and in specimen preparation for multiphoton microscopy are described. The transverse resolution is estimated, based on a photobleaching method. We also show that the performance of a multiphoton laser scanning microscope is significantly enhanced by the use of a confocal aperture.

AB - Two-photon laser scanning microscopy is discussed from a fluorophore and specimen point of view. Issues to be considered in the selection of the fluorophore and in specimen preparation for multiphoton microscopy are described. The transverse resolution is estimated, based on a photobleaching method. We also show that the performance of a multiphoton laser scanning microscope is significantly enhanced by the use of a confocal aperture.

KW - confocal microscopy

KW - fluorescence microscopy

KW - two-photon

UR - https://www.scopus.com/pages/publications/0030483548

U2 - 10.1002/1361-6374(199609)4:3<168::aid-bio7>3.0.co;2-8

DO - 10.1002/1361-6374(199609)4:3<168::aid-bio7>3.0.co;2-8

M3 - Article

AN - SCOPUS:0030483548

SN - 0966-9051

VL - 4

SP - 168

EP - 178

JO - Bioimaging

JF - Bioimaging

IS - 3

ER -

Two-photon laser scanning fluorescence microscopy - From a fluorophore and specimen perspective

Abstract

Keywords

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