Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine

Thomas D. Corso; German Torres; Christopher Goulah; Indrajit Roy; Angelo S. Gambino; John Nayda; Timothy Buckley; Ewa K. Stachowiak; Earl J. Bergey; Haridas Pudavar; Purnendu Dutta; David C. Bloom; William J. Bowers; Michal K. Stachowiak

doi:10.1016/j.molbrainres.2005.05.032

Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine

Thomas D. Corso
, German Torres
, Christopher Goulah
, Indrajit Roy
, Angelo S. Gambino
, John Nayda
, Timothy Buckley
, Ewa K. Stachowiak
, Earl J. Bergey
, Haridas Pudavar
, Purnendu Dutta
, David C. Bloom
, William J. Bowers
, Michal K. Stachowiak

Department of Pathology and Anatomical Sciences

SUNY Buffalo
Canisius College
New York Institute of Technology
University of Florida
University of Rochester

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

The effects of HSV-1 amplicon and polyethyleneimine (PEI)-mediated transfection of dominant negative FGF receptor-1 mutant FGFR1(TK-) into the rat brain substantia nigra (SN) were examined in vivo to model the reduced FGF signaling documented to occur in Parkinson's disease. The number of SN neurons that expressed tyrosine hydroxylase (TH) was significantly reduced following HSV-1 FGFR1(TK-) intranigral delivery and similar changes were observed after PEI-mediated FGFR1(TK-) transfections. Further, we also observed a significantly lower striatal dopamine content following the PEI transfection of FGFR1(TK-). Thus, we conclude that reduced FGF signaling in the SN of Parkinsonian patients could play a role in the impaired dopaminergic transmission associated with the degenerative disease.

Original language	English
Pages (from-to)	361-366
Number of pages	6
Journal	Molecular Brain Research
Volume	139
Issue number	2
DOIs	https://doi.org/10.1016/j.molbrainres.2005.05.032
State	Published - Oct 3 2005

Keywords

β-galactosidase
Fibroblast growth factor receptor
Gene transfection
Herpes simplex virus-1
Parkinson's disease
Polyethyleneimine
Substantia nigra

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Corso, T. D., Torres, G., Goulah, C., Roy, I., Gambino, A. S., Nayda, J., Buckley, T., Stachowiak, E. K., Bergey, E. J., Pudavar, H., Dutta, P., Bloom, D. C., Bowers, W. J., & Stachowiak, M. K. (2005). Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine. Molecular Brain Research, 139(2), 361-366. https://doi.org/10.1016/j.molbrainres.2005.05.032

@article{2694140cfc2448f6bce0d28523badfa3,

title = "Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine",

abstract = "The effects of HSV-1 amplicon and polyethyleneimine (PEI)-mediated transfection of dominant negative FGF receptor-1 mutant FGFR1(TK-) into the rat brain substantia nigra (SN) were examined in vivo to model the reduced FGF signaling documented to occur in Parkinson's disease. The number of SN neurons that expressed tyrosine hydroxylase (TH) was significantly reduced following HSV-1 FGFR1(TK-) intranigral delivery and similar changes were observed after PEI-mediated FGFR1(TK-) transfections. Further, we also observed a significantly lower striatal dopamine content following the PEI transfection of FGFR1(TK-). Thus, we conclude that reduced FGF signaling in the SN of Parkinsonian patients could play a role in the impaired dopaminergic transmission associated with the degenerative disease.",

keywords = "β-galactosidase, Fibroblast growth factor receptor, Gene transfection, Herpes simplex virus-1, Parkinson's disease, Polyethyleneimine, Substantia nigra",

author = "Corso, \{Thomas D.\} and German Torres and Christopher Goulah and Indrajit Roy and Gambino, \{Angelo S.\} and John Nayda and Timothy Buckley and Stachowiak, \{Ewa K.\} and Bergey, \{Earl J.\} and Haridas Pudavar and Purnendu Dutta and Bloom, \{David C.\} and Bowers, \{William J.\} and Stachowiak, \{Michal K.\}",

year = "2005",

month = oct,

day = "3",

doi = "10.1016/j.molbrainres.2005.05.032",

language = "English",

volume = "139",

pages = "361--366",

journal = "Molecular Brain Research",

issn = "0169-328X",

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Corso, TD, Torres, G, Goulah, C, Roy, I, Gambino, AS, Nayda, J, Buckley, T, Stachowiak, EK, Bergey, EJ, Pudavar, H, Dutta, P, Bloom, DC, Bowers, WJ & Stachowiak, MK 2005, 'Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine', Molecular Brain Research, vol. 139, no. 2, pp. 361-366. https://doi.org/10.1016/j.molbrainres.2005.05.032

Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine. / Corso, Thomas D.; Torres, German; Goulah, Christopher et al.
In: Molecular Brain Research, Vol. 139, No. 2, 03.10.2005, p. 361-366.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine

AU - Corso, Thomas D.

AU - Torres, German

AU - Goulah, Christopher

AU - Roy, Indrajit

AU - Gambino, Angelo S.

AU - Nayda, John

AU - Buckley, Timothy

AU - Stachowiak, Ewa K.

AU - Bergey, Earl J.

AU - Pudavar, Haridas

AU - Dutta, Purnendu

AU - Bloom, David C.

AU - Bowers, William J.

AU - Stachowiak, Michal K.

PY - 2005/10/3

Y1 - 2005/10/3

N2 - The effects of HSV-1 amplicon and polyethyleneimine (PEI)-mediated transfection of dominant negative FGF receptor-1 mutant FGFR1(TK-) into the rat brain substantia nigra (SN) were examined in vivo to model the reduced FGF signaling documented to occur in Parkinson's disease. The number of SN neurons that expressed tyrosine hydroxylase (TH) was significantly reduced following HSV-1 FGFR1(TK-) intranigral delivery and similar changes were observed after PEI-mediated FGFR1(TK-) transfections. Further, we also observed a significantly lower striatal dopamine content following the PEI transfection of FGFR1(TK-). Thus, we conclude that reduced FGF signaling in the SN of Parkinsonian patients could play a role in the impaired dopaminergic transmission associated with the degenerative disease.

AB - The effects of HSV-1 amplicon and polyethyleneimine (PEI)-mediated transfection of dominant negative FGF receptor-1 mutant FGFR1(TK-) into the rat brain substantia nigra (SN) were examined in vivo to model the reduced FGF signaling documented to occur in Parkinson's disease. The number of SN neurons that expressed tyrosine hydroxylase (TH) was significantly reduced following HSV-1 FGFR1(TK-) intranigral delivery and similar changes were observed after PEI-mediated FGFR1(TK-) transfections. Further, we also observed a significantly lower striatal dopamine content following the PEI transfection of FGFR1(TK-). Thus, we conclude that reduced FGF signaling in the SN of Parkinsonian patients could play a role in the impaired dopaminergic transmission associated with the degenerative disease.

KW - β-galactosidase

KW - Fibroblast growth factor receptor

KW - Gene transfection

KW - Herpes simplex virus-1

KW - Parkinson's disease

KW - Polyethyleneimine

KW - Substantia nigra

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DO - 10.1016/j.molbrainres.2005.05.032

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C2 - 16039006

AN - SCOPUS:24744448035

SN - 0169-328X

VL - 139

SP - 361

EP - 366

JO - Molecular Brain Research

JF - Molecular Brain Research

IS - 2

ER -

Transfection of tyrosine kinase deleted FGF receptor-1 into rat brain substantia nigra reduces the number of tyrosine hydroxylase expressing neurons and decreases concentration levels of striatal dopamine

Abstract

Keywords

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