Three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor determined at 2.5 Å resolution from X-ray data for a twin crystal

P. V. Afonin; A. V. Fokin; L. N. Shingarova; V. G. Korobko; I. N. Tsygannik; I. V. Artem’ev; S. V. Pletnev; W. Pangborn; W. L. Duax; V. Z. Pletnev

doi:10.1134/1.1496062

Three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor determined at 2.5 Å resolution from X-ray data for a twin crystal

P. V. Afonin
, A. V. Fokin
, L. N. Shingarova
, V. G. Korobko
, I. N. Tsygannik
, I. V. Artem’ev
, S. V. Pletnev
, W. Pangborn
, W. L. Duax
, V. Z. Pletnev

Department of Structural Biology

Russian Academy of Sciences
Shubnikov Institute of Crystallography, Russian Academy of Sciences
Hauptman-Woodward Medical Research Institute, Inc.

Research output: Contribution to journal › Article › peer-review

Abstract

The three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor (TNF-α) was established at 2.5 Å resolution by the molecular replacement method. The crystals of the mutant belong to sp. gr. R3. The specimen has a hemihedral twinning fraction of approximately one half with the twin law corresponding to an additional twofold axis along the a- or b-axis of the crystal lattice. The model analysis of interactions between functionally important loop 29-36 of the mutant and the receptors p55 and p75 was performed.

Original language	English
Pages (from-to)	629-634
Number of pages	6
Journal	Crystallography Reports
Volume	47
Issue number	4
DOIs	https://doi.org/10.1134/1.1496062
State	Published - 2002

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Afonin, P. V., Fokin, A. V., Shingarova, L. N., Korobko, V. G., Tsygannik, I. N., Artem’ev, I. V., Pletnev, S. V., Pangborn, W., Duax, W. L., & Pletnev, V. Z. (2002). Three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor determined at 2.5 Å resolution from X-ray data for a twin crystal. Crystallography Reports, 47(4), 629-634. https://doi.org/10.1134/1.1496062

@article{cf0bdf4442ca427b94236c17f289b912,

title = "Three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor determined at 2.5 {\AA} resolution from X-ray data for a twin crystal",

abstract = "The three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor (TNF-α) was established at 2.5 {\AA} resolution by the molecular replacement method. The crystals of the mutant belong to sp. gr. R3. The specimen has a hemihedral twinning fraction of approximately one half with the twin law corresponding to an additional twofold axis along the a- or b-axis of the crystal lattice. The model analysis of interactions between functionally important loop 29-36 of the mutant and the receptors p55 and p75 was performed.",

author = "Afonin, \{P. V.\} and Fokin, \{A. V.\} and Shingarova, \{L. N.\} and Korobko, \{V. G.\} and Tsygannik, \{I. N.\} and Artem{\textquoteright}ev, \{I. V.\} and Pletnev, \{S. V.\} and W. Pangborn and Duax, \{W. L.\} and Pletnev, \{V. Z.\}",

year = "2002",

doi = "10.1134/1.1496062",

language = "English",

volume = "47",

pages = "629--634",

journal = "Crystallography Reports",

issn = "1063-7745",

publisher = "Pleiades Publishing",

number = "4",

}

Afonin, PV, Fokin, AV, Shingarova, LN, Korobko, VG, Tsygannik, IN, Artem’ev, IV, Pletnev, SV, Pangborn, W, Duax, WL & Pletnev, VZ 2002, 'Three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor determined at 2.5 Å resolution from X-ray data for a twin crystal', Crystallography Reports, vol. 47, no. 4, pp. 629-634. https://doi.org/10.1134/1.1496062

TY - JOUR

T1 - Three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor determined at 2.5 Å resolution from X-ray data for a twin crystal

AU - Afonin, P. V.

AU - Fokin, A. V.

AU - Shingarova, L. N.

AU - Korobko, V. G.

AU - Tsygannik, I. N.

AU - Artem’ev, I. V.

AU - Pletnev, S. V.

AU - Pangborn, W.

AU - Duax, W. L.

AU - Pletnev, V. Z.

PY - 2002

Y1 - 2002

N2 - The three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor (TNF-α) was established at 2.5 Å resolution by the molecular replacement method. The crystals of the mutant belong to sp. gr. R3. The specimen has a hemihedral twinning fraction of approximately one half with the twin law corresponding to an additional twofold axis along the a- or b-axis of the crystal lattice. The model analysis of interactions between functionally important loop 29-36 of the mutant and the receptors p55 and p75 was performed.

AB - The three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor (TNF-α) was established at 2.5 Å resolution by the molecular replacement method. The crystals of the mutant belong to sp. gr. R3. The specimen has a hemihedral twinning fraction of approximately one half with the twin law corresponding to an additional twofold axis along the a- or b-axis of the crystal lattice. The model analysis of interactions between functionally important loop 29-36 of the mutant and the receptors p55 and p75 was performed.

UR - https://www.scopus.com/pages/publications/0036652599

U2 - 10.1134/1.1496062

DO - 10.1134/1.1496062

M3 - Article

AN - SCOPUS:0036652599

SN - 1063-7745

VL - 47

SP - 629

EP - 634

JO - Crystallography Reports

JF - Crystallography Reports

IS - 4

ER -

Three-dimensional structure of the Arg32His mutant of the human tumor necrosis factor determined at 2.5 Å resolution from X-ray data for a twin crystal

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