Three-dimensional confocal thermal imaging using anti-Stokes luminescence

A. V. Kachynski; A. N. Kuzmin; H. E. Pudavar; P. N. Prasad

doi:10.1063/1.1993761

Three-dimensional confocal thermal imaging using anti-Stokes luminescence

A. V. Kachynski
, A. N. Kuzmin
, H. E. Pudavar
, P. N. Prasad

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

High-resolution thermal images of dye-stained live cells are obtained using confocal anti-Stokes luminescence on a confocal laser scanning microscope. Stokes and anti-Stokes fluorescence signal ratio imaging, using dual laser excitation with a fast alternate line scan, is used to monitor dynamical temperature changes at submicrometer resolution. Temperature mapping of a dye-doped polymer film with local heating is shown as an example. Three-dimensional mapping of local temperature changes in living cells induced by an external laser as the heating source, is obtained with temperature resolution better than 0.5 K.

Original language	English
Article number	023901
Journal	Applied Physics Letters
Volume	87
Issue number	2
DOIs	https://doi.org/10.1063/1.1993761
State	Published - Jul 11 2005

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title = "Three-dimensional confocal thermal imaging using anti-Stokes luminescence",

abstract = "High-resolution thermal images of dye-stained live cells are obtained using confocal anti-Stokes luminescence on a confocal laser scanning microscope. Stokes and anti-Stokes fluorescence signal ratio imaging, using dual laser excitation with a fast alternate line scan, is used to monitor dynamical temperature changes at submicrometer resolution. Temperature mapping of a dye-doped polymer film with local heating is shown as an example. Three-dimensional mapping of local temperature changes in living cells induced by an external laser as the heating source, is obtained with temperature resolution better than 0.5 K.",

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AU - Kachynski, A. V.

AU - Kuzmin, A. N.

AU - Pudavar, H. E.

AU - Prasad, P. N.

PY - 2005/7/11

Y1 - 2005/7/11

N2 - High-resolution thermal images of dye-stained live cells are obtained using confocal anti-Stokes luminescence on a confocal laser scanning microscope. Stokes and anti-Stokes fluorescence signal ratio imaging, using dual laser excitation with a fast alternate line scan, is used to monitor dynamical temperature changes at submicrometer resolution. Temperature mapping of a dye-doped polymer film with local heating is shown as an example. Three-dimensional mapping of local temperature changes in living cells induced by an external laser as the heating source, is obtained with temperature resolution better than 0.5 K.

AB - High-resolution thermal images of dye-stained live cells are obtained using confocal anti-Stokes luminescence on a confocal laser scanning microscope. Stokes and anti-Stokes fluorescence signal ratio imaging, using dual laser excitation with a fast alternate line scan, is used to monitor dynamical temperature changes at submicrometer resolution. Temperature mapping of a dye-doped polymer film with local heating is shown as an example. Three-dimensional mapping of local temperature changes in living cells induced by an external laser as the heating source, is obtained with temperature resolution better than 0.5 K.

UR - https://www.scopus.com/pages/publications/24144487082

U2 - 10.1063/1.1993761

DO - 10.1063/1.1993761

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VL - 87

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 2

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Three-dimensional confocal thermal imaging using anti-Stokes luminescence

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