The use of high-pressure liquid chromatography in the simultaneous assay of 11β-hydroxylase and 18-hydroxylase in zona fascloulata-retioularis tissue of the rat adrenal cortex

Samuel Gallant; Sally M. Bruckheimer; Alexander C. Brownie

doi:10.1016/0003-2697(78)90741-8

The use of high-pressure liquid chromatography in the simultaneous assay of 11β-hydroxylase and 18-hydroxylase in zona fascloulata-retioularis tissue of the rat adrenal cortex

Samuel Gallant
, Sally M. Bruckheimer
, Alexander C. Brownie

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

An isocratic, reverse-phase system for determining adrenal cortical steroids by high-pressure liquid chromatography is described. The method enables the rapid, accurate, and precise separation and quantitation of the major steroids produced during in vitro incubations of rat adrenal cortex mitochondria with 11-deoxycorticosterone. The application of this method to the determination of 11β- and 18-hydroxylase activity in the presence of metopirone is illustrated, and its utility for studying other enzymatic reactions in the adrenal gland is discussed.

Original language	English
Pages (from-to)	196-202
Number of pages	7
Journal	Analytical Biochemistry
Volume	89
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(78)90741-8
State	Published - Aug 15 1978

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10.1016/0003-2697(78)90741-8

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abstract = "An isocratic, reverse-phase system for determining adrenal cortical steroids by high-pressure liquid chromatography is described. The method enables the rapid, accurate, and precise separation and quantitation of the major steroids produced during in vitro incubations of rat adrenal cortex mitochondria with 11-deoxycorticosterone. The application of this method to the determination of 11β- and 18-hydroxylase activity in the presence of metopirone is illustrated, and its utility for studying other enzymatic reactions in the adrenal gland is discussed.",

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The use of high-pressure liquid chromatography in the simultaneous assay of 11β-hydroxylase and 18-hydroxylase in zona fascloulata-retioularis tissue of the rat adrenal cortex. / Gallant, Samuel; Bruckheimer, Sally M.; Brownie, Alexander C.
In: Analytical Biochemistry, Vol. 89, No. 1, 15.08.1978, p. 196-202.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The use of high-pressure liquid chromatography in the simultaneous assay of 11β-hydroxylase and 18-hydroxylase in zona fascloulata-retioularis tissue of the rat adrenal cortex

AU - Gallant, Samuel

AU - Bruckheimer, Sally M.

AU - Brownie, Alexander C.

PY - 1978/8/15

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N2 - An isocratic, reverse-phase system for determining adrenal cortical steroids by high-pressure liquid chromatography is described. The method enables the rapid, accurate, and precise separation and quantitation of the major steroids produced during in vitro incubations of rat adrenal cortex mitochondria with 11-deoxycorticosterone. The application of this method to the determination of 11β- and 18-hydroxylase activity in the presence of metopirone is illustrated, and its utility for studying other enzymatic reactions in the adrenal gland is discussed.

AB - An isocratic, reverse-phase system for determining adrenal cortical steroids by high-pressure liquid chromatography is described. The method enables the rapid, accurate, and precise separation and quantitation of the major steroids produced during in vitro incubations of rat adrenal cortex mitochondria with 11-deoxycorticosterone. The application of this method to the determination of 11β- and 18-hydroxylase activity in the presence of metopirone is illustrated, and its utility for studying other enzymatic reactions in the adrenal gland is discussed.

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DO - 10.1016/0003-2697(78)90741-8

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The use of high-pressure liquid chromatography in the simultaneous assay of 11β-hydroxylase and 18-hydroxylase in zona fascloulata-retioularis tissue of the rat adrenal cortex

Abstract

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