The structure of a complex of bovine α-thrombin and recombinant hirudin at 2.8-Å resolution

Crystals of the complex of bovine α-thrombin with recombinant hirudin variant 1 have space group C222₁ with cell constants a = 59.11, b = 102.62, and c = 143.26 Å. The orientation and position of the thrombin component was determined by molecular replacement and the hirudin molecule was fit in 2|F_o| - |F_c| electron density maps. The structure was refined by restrained least squares and simulated annealing to R = 0.161 at 2.8-Å resolution. The binding of hirudin to thrombin is generally similar to that observed in the crystals of human thrombin-hirudin. Several differences in the interactions of the COOH-terminal polypeptide of hirudin, specifically of residues Asp-55h, Phe-56h, Glu-57h, and Glu-58h, and a few differences in the interactions of the hirudin core, specifically of residues Asp-5h, Ser-19h, and Asn-20h, with thrombin from human thrombin-hirudin suggest that there is some flexibility in the binding of these 2 molecules. Most of the residues in the 9 subsites that bind fibrinopeptide A_7-16 to thrombin also interact with the NH₂-terminal domain of hirudin. The S1 subsite is a notable exception in that only 1 of its 6 residues, namely Ser-214, interacts with hirudin. The only difference between human and bovine thrombins that appears to influence the binding of hirudin is the replacement of Lys-149E by an acidic glutamate in the bovine enzyme.