The role of calcium in turgor regulation in Chara longifolia

The salt‐tolerant alga Chara longifolia (Robinson) is capable of regulating its turgor in response to hypotonic stress resulting from a decrease in the osmotic pressure of the medium. This regulatory process takes only 40 min in small cells (length ≤ 10 mm), but requires 3d in large cells (length ≥30mm). Turgor regulation in small cells is comprised of two phases, a fast phase reducing the increased turgor by about 25% in the First 5 min, and a second phase reducing the turgor to near the original value within 40 min. The second phase is inhibited by reducing the concentration of Ca²⁺ in the external medium from 4.6 to 0.01 mol m⁻³; the first phase is less affected by the reduction of Ca²⁺. In the first 5 min of stress, the membrane depolarizes in a voltage‐dependent fashion, electrical conductance of the membrane increases transiently and cytoplasmic streaming is inhibited. When the external Ca²⁺ concentration is lowered, conductance does not increase and streaming continues unaffected. In a low ionic strength medium, Ca²⁺ is not required in the medium for turgor regulation. To test the hypothesis that there is increased Ca²⁺ entry from the medium during turgor regulation, we measured the influx of ⁴⁵Ca²⁺ into the cell. We found an increased influx of Ca²⁺, from 18 to 36 nmol m⁻² s⁻¹ during the first 30 to 90 s following osmotic stress. This increase was evident only in cells below about 7 mm in length, and was more marked in smaller cells.