The protection of Tiron to the injury of organ of Corti cultured in vitro caused by exogenous H2O2 in mouse

OBJECTIVE: In order to observe the action of H2O2 on inner and outer hair cells of organ of Corti in mouse, and discuss whether Tiron can effectively resistant the injury of free radicals in cultured cochlea in vitro or not. METHOD: Using the culture technology of organ of Corti in newborn mouse in vitro, established the model of the injury of inner and outer hair cells caused by exogenous H2O2. And observed the protection to the damage by H2O2 at different concentration of Tiron. RESULT: When the concentration of H2O2 is higher than 1.0 mmol/L, the loss of hair cells in the bottom, middle, and parietal turn of basilar membrane vary, the injury of bottom turn is more severe. In lower than 0.5 mmol/L group, the injury of hair cells is no relationship with the location. Adding Tiron (10 mmol/L) in culture can apparently decrease the injury of hair cells caused by H2O2. When the concentration of H2O2 is 0.01-1.0 mmol/L, Tiron can almost inhibit the loss of hair cells completely. CONCLUSION: There is apparently preventive effection of Tiron on organ of Corti cultured in vitro, and the mechanism of inhibiting injury caused by H2O2 is probably by clearing O2- and combining irony ion.