The preparation, crystallization, and preliminary X-ray study of a fab-fragment of monoclonal antibody to human interleukin-2 and its complex with an antigenic peptide

I. Yu Mikhailova; T. Yu Mareeva; I. N. Tsygannik; I. I. Mikhaleva; L. V. Onoprienko; A. A. Vikhrov; E. A. Markvicheva; W. Pangborn; W. Duax; V. A. Nesmeyanov; V. Z. Pletnev

The preparation, crystallization, and preliminary X-ray study of a fab-fragment of monoclonal antibody to human interleukin-2 and its complex with an antigenic peptide

I. Yu Mikhailova
, T. Yu Mareeva
, I. N. Tsygannik
, I. I. Mikhaleva
, L. V. Onoprienko
, A. A. Vikhrov
, E. A. Markvicheva
, W. Pangborn
, W. Duax
, V. A. Nesmeyanov
, V. Z. Pletnev

Department of Structural Biology

Russian Academy of Sciences
Hauptman-Woodward Medical Research Institute, Inc.

Research output: Contribution to journal › Article › peer-review

Abstract

Antigen-binding fragments (Fab) of mouse monoclonal antibodies to human interleukin-2 were obtained in preparative quantities by a modified procedure. These Fab-fragments were shown to be homogeneous according to the isoelectric focusing method. Various monocrystals of these free Fab-fragments and their complexes with the antigenic peptide corresponding to the 59-72 sequence of interleukin-2 were obtained. These were shown to be suitable for X-ray and were preliminarily studied by X-ray.

Original language	English
Pages (from-to)	252
Number of pages	1
Journal	Bioorganicheskaya Khimiya
Volume	25
Issue number	4
State	Published - 1999

Keywords

Crystallization
Fab-fragment
Interleukin-2
Monoclonal antibody
X-ray analysis

Cite this

Mikhailova, I. Y., Mareeva, T. Y., Tsygannik, I. N., Mikhaleva, I. I., Onoprienko, L. V., Vikhrov, A. A., Markvicheva, E. A., Pangborn, W., Duax, W., Nesmeyanov, V. A., & Pletnev, V. Z. (1999). The preparation, crystallization, and preliminary X-ray study of a fab-fragment of monoclonal antibody to human interleukin-2 and its complex with an antigenic peptide. Bioorganicheskaya Khimiya, 25(4), 252.

@article{382d088017b04703a9924b4cb4b74a0d,

title = "The preparation, crystallization, and preliminary X-ray study of a fab-fragment of monoclonal antibody to human interleukin-2 and its complex with an antigenic peptide",

abstract = "Antigen-binding fragments (Fab) of mouse monoclonal antibodies to human interleukin-2 were obtained in preparative quantities by a modified procedure. These Fab-fragments were shown to be homogeneous according to the isoelectric focusing method. Various monocrystals of these free Fab-fragments and their complexes with the antigenic peptide corresponding to the 59-72 sequence of interleukin-2 were obtained. These were shown to be suitable for X-ray and were preliminarily studied by X-ray.",

keywords = "Crystallization, Fab-fragment, Interleukin-2, Monoclonal antibody, X-ray analysis",

author = "Mikhailova, \{I. Yu\} and Mareeva, \{T. Yu\} and Tsygannik, \{I. N.\} and Mikhaleva, \{I. I.\} and Onoprienko, \{L. V.\} and Vikhrov, \{A. A.\} and Markvicheva, \{E. A.\} and W. Pangborn and W. Duax and Nesmeyanov, \{V. A.\} and Pletnev, \{V. Z.\}",

year = "1999",

language = "English",

volume = "25",

pages = "252",

journal = "Bioorganicheskaya Khimiya",

issn = "0132-3423",

publisher = "Izdatel'stvo Nauka",

number = "4",

}

Mikhailova, IY, Mareeva, TY, Tsygannik, IN, Mikhaleva, II, Onoprienko, LV, Vikhrov, AA, Markvicheva, EA, Pangborn, W, Duax, W, Nesmeyanov, VA & Pletnev, VZ 1999, 'The preparation, crystallization, and preliminary X-ray study of a fab-fragment of monoclonal antibody to human interleukin-2 and its complex with an antigenic peptide', Bioorganicheskaya Khimiya, vol. 25, no. 4, pp. 252.

TY - JOUR

T1 - The preparation, crystallization, and preliminary X-ray study of a fab-fragment of monoclonal antibody to human interleukin-2 and its complex with an antigenic peptide

AU - Mikhailova, I. Yu

AU - Mareeva, T. Yu

AU - Tsygannik, I. N.

AU - Mikhaleva, I. I.

AU - Onoprienko, L. V.

AU - Vikhrov, A. A.

AU - Markvicheva, E. A.

AU - Pangborn, W.

AU - Duax, W.

AU - Nesmeyanov, V. A.

AU - Pletnev, V. Z.

PY - 1999

Y1 - 1999

N2 - Antigen-binding fragments (Fab) of mouse monoclonal antibodies to human interleukin-2 were obtained in preparative quantities by a modified procedure. These Fab-fragments were shown to be homogeneous according to the isoelectric focusing method. Various monocrystals of these free Fab-fragments and their complexes with the antigenic peptide corresponding to the 59-72 sequence of interleukin-2 were obtained. These were shown to be suitable for X-ray and were preliminarily studied by X-ray.

AB - Antigen-binding fragments (Fab) of mouse monoclonal antibodies to human interleukin-2 were obtained in preparative quantities by a modified procedure. These Fab-fragments were shown to be homogeneous according to the isoelectric focusing method. Various monocrystals of these free Fab-fragments and their complexes with the antigenic peptide corresponding to the 59-72 sequence of interleukin-2 were obtained. These were shown to be suitable for X-ray and were preliminarily studied by X-ray.

KW - Crystallization

KW - Fab-fragment

KW - Interleukin-2

KW - Monoclonal antibody

KW - X-ray analysis

UR - https://www.scopus.com/pages/publications/0039626164

M3 - Article

AN - SCOPUS:0039626164

SN - 0132-3423

VL - 25

SP - 252

JO - Bioorganicheskaya Khimiya

JF - Bioorganicheskaya Khimiya

IS - 4

ER -

The preparation, crystallization, and preliminary X-ray study of a fab-fragment of monoclonal antibody to human interleukin-2 and its complex with an antigenic peptide

Abstract

Keywords

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