TY - GEN
T1 - The optical biomedical sensors for the DNA detection and imaging based on two-photon excited luminescent styryl dyes. Phototoxic influence on the DNA
AU - Yashchuk, Valeriy M.
AU - Kudrya, Vladyslav Yu
AU - Losytskyy, Mykhaylo Yu
AU - Tokar, Valentyna P.
AU - Yarmoluk, Sergiy M.
AU - Dmytruk, Igor M.
AU - Prokopets, Vadym M.
AU - Kovalska, Vladyslava B.
AU - Balanda, Anatoliy O.
AU - Kryvorotenko, Dmytro V.
AU - Ogul'chansky, Tymish Yu
PY - 2008
Y1 - 2008
N2 - The optical absorption, fluorescence and phosphorescence of the novel styryl dyes developed for the fluorescent detection of DNA were investigated. The energy structures of dye molecules as well as spectral manifestations of the dyes aggregate formation and interaction with DNA were studied. The dramatic increase (up to 1000 times) of the fluorescence intensity of dyes in the presence of DNA was observed. The photostability and phototoxic influence on the DNA of several styryl dyes were studied by analyzing absorption, fluorescence and phosphorescence spectra of these dyes and dye-DNA systems. Changes of the optical density value of dye-DNA solutions caused by the visible light irradiation were fixed in the wavelength regions of the DNA absorption and of the dye absorption. Fluorescence emission of dye-DNA complexes upon two-photon excitation (TPE) at wavelength 1064 nm with the 20 ns pulsed YAG: Nd 3+ laser and at 840 nm with the 90 fs pulsed Ti:sapphire laser was registered. The values of two-photon absorption cross-sections of dye-DNA complexes were evaluated.
AB - The optical absorption, fluorescence and phosphorescence of the novel styryl dyes developed for the fluorescent detection of DNA were investigated. The energy structures of dye molecules as well as spectral manifestations of the dyes aggregate formation and interaction with DNA were studied. The dramatic increase (up to 1000 times) of the fluorescence intensity of dyes in the presence of DNA was observed. The photostability and phototoxic influence on the DNA of several styryl dyes were studied by analyzing absorption, fluorescence and phosphorescence spectra of these dyes and dye-DNA systems. Changes of the optical density value of dye-DNA solutions caused by the visible light irradiation were fixed in the wavelength regions of the DNA absorption and of the dye absorption. Fluorescence emission of dye-DNA complexes upon two-photon excitation (TPE) at wavelength 1064 nm with the 20 ns pulsed YAG: Nd 3+ laser and at 840 nm with the 90 fs pulsed Ti:sapphire laser was registered. The values of two-photon absorption cross-sections of dye-DNA complexes were evaluated.
KW - DNA detection
KW - Fluorescent probes
KW - Photostability
KW - Phototoxicity
KW - Two-photon excitation
UR - https://www.scopus.com/pages/publications/43149118378
U2 - 10.1117/12.778096
DO - 10.1117/12.778096
M3 - Conference contribution
AN - SCOPUS:43149118378
SN - 9780819469625
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Photonics North 2007
T2 - Photonics North 2007
Y2 - 4 June 2008 through 7 June 2008
ER -