The contrast formation in optical microscopy

Ping Chin Cheng

doi:10.1007/978-0-387-45524-2_8

The contrast formation in optical microscopy

Ping Chin Cheng

Department of Electrical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

42 Scopus citations

Abstract

In any form of microscopy, one needs not only an imaging system with enough resolution to delineate the fine details of the specimen but also a suitable contrast mechanism by which to see the shape of the structures of interest. Contrast is the difference between the signal in one pixel and that in another that conveys to the viewer information about the shape of the specimen. It is the difference between a blank screen and an image.

Original language	English
Title of host publication	Handbook of Biological Confocal Microscopy
Subtitle of host publication	Third Edition
Publisher	Springer US
Pages	162-206
Number of pages	45
ISBN (Print)	038725921X, 9780387259215
DOIs	https://doi.org/10.1007/978-0-387-45524-2_8
State	Published - 2006

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title = "The contrast formation in optical microscopy",

abstract = "In any form of microscopy, one needs not only an imaging system with enough resolution to delineate the fine details of the specimen but also a suitable contrast mechanism by which to see the shape of the structures of interest. Contrast is the difference between the signal in one pixel and that in another that conveys to the viewer information about the shape of the specimen. It is the difference between a blank screen and an image.",

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AB - In any form of microscopy, one needs not only an imaging system with enough resolution to delineate the fine details of the specimen but also a suitable contrast mechanism by which to see the shape of the structures of interest. Contrast is the difference between the signal in one pixel and that in another that conveys to the viewer information about the shape of the specimen. It is the difference between a blank screen and an image.

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The contrast formation in optical microscopy

Abstract

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