The 5’ Ends of Yeast Killer Factor RNAs Are PPPGP

Jeremy Bruenn; Bernadette Keitz

doi:10.1093/nar/3.10.2427

The 5’ Ends of Yeast Killer Factor RNAs Are PPPGP

Jeremy Bruenn
, Bernadette Keitz

Biological Sciences

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

36 Scopus citations

Abstract

The 5’ nucleotides of the double-stranded RNAs of yeast killer factor have been isolated by digestion with pancreatic, T₁ and T₂ RNase followed by two-dimensional electrophoresis. They were identified by bacterial alkaline phosphatase and snake venom phosphodiesterase digestions. Both the larger double-stranded RNA (L, of 2.5 × 106 daltons) and the smaller double-stranded RNA (M, of 1.4 × 10⁶ daltons) have the 5’ end groups pppGp. These 5’ ends are dissimilar to those of the double-stranded RNAs of animal viruses but may be characteristic of the 5’ ends of the double stranded RNAs of fungal viruses.

Original language	English
Pages (from-to)	2427-2436
Number of pages	10
Journal	Nucleic Acids Research
Volume	3
Issue number	10
DOIs	https://doi.org/10.1093/nar/3.10.2427
State	Published - Oct 1976

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title = "The 5{\textquoteright} Ends of Yeast Killer Factor RNAs Are PPPGP",

abstract = "The 5{\textquoteright} nucleotides of the double-stranded RNAs of yeast killer factor have been isolated by digestion with pancreatic, T1 and T2 RNase followed by two-dimensional electrophoresis. They were identified by bacterial alkaline phosphatase and snake venom phosphodiesterase digestions. Both the larger double-stranded RNA (L, of 2.5 × 106 daltons) and the smaller double-stranded RNA (M, of 1.4 × 106 daltons) have the 5{\textquoteright} end groups pppGp. These 5{\textquoteright} ends are dissimilar to those of the double-stranded RNAs of animal viruses but may be characteristic of the 5{\textquoteright} ends of the double stranded RNAs of fungal viruses.",

author = "Jeremy Bruenn and Bernadette Keitz",

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AU - Keitz, Bernadette

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N2 - The 5’ nucleotides of the double-stranded RNAs of yeast killer factor have been isolated by digestion with pancreatic, T1 and T2 RNase followed by two-dimensional electrophoresis. They were identified by bacterial alkaline phosphatase and snake venom phosphodiesterase digestions. Both the larger double-stranded RNA (L, of 2.5 × 106 daltons) and the smaller double-stranded RNA (M, of 1.4 × 106 daltons) have the 5’ end groups pppGp. These 5’ ends are dissimilar to those of the double-stranded RNAs of animal viruses but may be characteristic of the 5’ ends of the double stranded RNAs of fungal viruses.

AB - The 5’ nucleotides of the double-stranded RNAs of yeast killer factor have been isolated by digestion with pancreatic, T1 and T2 RNase followed by two-dimensional electrophoresis. They were identified by bacterial alkaline phosphatase and snake venom phosphodiesterase digestions. Both the larger double-stranded RNA (L, of 2.5 × 106 daltons) and the smaller double-stranded RNA (M, of 1.4 × 106 daltons) have the 5’ end groups pppGp. These 5’ ends are dissimilar to those of the double-stranded RNAs of animal viruses but may be characteristic of the 5’ ends of the double stranded RNAs of fungal viruses.

UR - https://www.scopus.com/pages/publications/0017142871

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ER -

The 5’ Ends of Yeast Killer Factor RNAs Are PPPGP

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