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Testing the Ability of Compounds to Induce Swarming

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

One of the most distinctive features of Proteus mirabilis is its ability to undergo differentiation from short, rod-shaped vegetative cells with peritrichous flagella to massively elongated swarm cells that express hundreds to thousands of flagella. The unique bull’s-eye pattern that forms from cycles of active swarming and consolidation back to the vegetative state has long been a distinguishing characteristic of this species. Many factors involved in regulation of flagellar synthesis and swarm cell differentiation have been characterized, but the exact conditions sensed by P. mirabilis that send a signal to initiate differentiation and motility have yet to be fully elucidated. Here we describe a method for using several types of media to investigate compounds that induce swarming motility under conditions that would not normally be permissive.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages27-34
Number of pages8
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume2021
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Amino acids
  • Humidity
  • Motility
  • pH
  • Surface tension
  • Swarming

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