TY - JOUR
T1 - Systemic treatment with resveratrol reduces the progression of experimental periodontitis and arthritis in rats
AU - Correâ, Monica G.
AU - Pires, Paula Rodrigues
AU - Ribeiro, Fernanda Vieira
AU - Pimentel, Suzana Peres
AU - Cirano, Fabiano Ribeiro
AU - Napimoga, Marcelo Henrique
AU - Casati, Marcio Z.
AU - Casarin, Renato Correâ Viana
N1 - Publisher Copyright:
© 2018 Correâ et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2018/10
Y1 - 2018/10
N2 - Rheumatoid arthritis and periodontitis are chronic inflammatory diseases which has been closely associated due to the nature of immune-inflammatory imbalance response. Resveratrol is a naturall product with biological proprieties that may promote immunomodulatory effects on host response. This study investigated resveratrol continuous administration effect on experimental periodontitis and arthritis progression in rats. Thirty-five rats were assigned to the following groups: 1experimental arthritis + experimental periodontitis + placebo (RA+EP +PL) (n = 12); 2 ±RA+EP+ ibuprofen (RA+PE+IB) (n = 11); 3RA+EP+ resveratrol (RA+PE+RSV) (n = 11). After euthanasia, the specimens were processed for morphometric analysis of bone loss, and the gingival tissue surrounding the first molar was collected for quantification of inflammatory markers using a Luminex/MAGpix assay and anti-citrullinated protein antibody (ACCPA) levels were measured by ELISA assay. Serum level of rheumatoid factor (RF) was measured by ELISA assay. Paw edema was analyzed using a plethysmometer. Higher bone loss was observed in PL group, when compared to IB and RSV groups. RSV group presented higher IL-4 concentration than PL and IB groups. Resveratrol reduced RF serum levels and both IB and RSV decreased ACCPA gingival levels. Besides, paw swelling level was significantly lower in IB and RSV groups in the 21th day and only in RSV group in the 28th day. Histological analyzes showed smooth articular surface and higher width of the subchondral cortical in RSV group. Resveratrol showed modulatory effect and seems to reduce the inflammatory signs of arthritis and articular damage throughout the time.
AB - Rheumatoid arthritis and periodontitis are chronic inflammatory diseases which has been closely associated due to the nature of immune-inflammatory imbalance response. Resveratrol is a naturall product with biological proprieties that may promote immunomodulatory effects on host response. This study investigated resveratrol continuous administration effect on experimental periodontitis and arthritis progression in rats. Thirty-five rats were assigned to the following groups: 1experimental arthritis + experimental periodontitis + placebo (RA+EP +PL) (n = 12); 2 ±RA+EP+ ibuprofen (RA+PE+IB) (n = 11); 3RA+EP+ resveratrol (RA+PE+RSV) (n = 11). After euthanasia, the specimens were processed for morphometric analysis of bone loss, and the gingival tissue surrounding the first molar was collected for quantification of inflammatory markers using a Luminex/MAGpix assay and anti-citrullinated protein antibody (ACCPA) levels were measured by ELISA assay. Serum level of rheumatoid factor (RF) was measured by ELISA assay. Paw edema was analyzed using a plethysmometer. Higher bone loss was observed in PL group, when compared to IB and RSV groups. RSV group presented higher IL-4 concentration than PL and IB groups. Resveratrol reduced RF serum levels and both IB and RSV decreased ACCPA gingival levels. Besides, paw swelling level was significantly lower in IB and RSV groups in the 21th day and only in RSV group in the 28th day. Histological analyzes showed smooth articular surface and higher width of the subchondral cortical in RSV group. Resveratrol showed modulatory effect and seems to reduce the inflammatory signs of arthritis and articular damage throughout the time.
UR - https://www.scopus.com/pages/publications/85054468858
U2 - 10.1371/journal.pone.0204414
DO - 10.1371/journal.pone.0204414
M3 - Article
C2 - 30281626
AN - SCOPUS:85054468858
SN - 1932-6203
VL - 13
JO - PLOS ONE
JF - PLOS ONE
IS - 10
M1 - e0204414
ER -