Synthetic antigens as immunogens: Part IV. Specificity of antisera developed to gal β1-3(glcNAc β1-6)galNAc and glcNAc βl-6galNAc

Antisera to the chemically synthesized trisaccharide, Gal β1-3(GlcNAc β1-6)GalNAc, and disaccharide, GlcNAc β1-6GalNAc, were developed using the diazophenyl bovine serum albumin derivatives. The binding specificity of the antisera were analyzed by enzyme immunoassays with structurally related, chemically synthesized oligosaccharides. The anti-trisaccharide antibody showed no reactivity to T antigenic structures which bear the Gal pl-3GalNAc moiety. The immunodominant area of the Gral β1-3(GlcNAc β 1-6)GalNAc was contained in the GlcNAc β1-6GalNAc region of the molecule. The reactivity pattern of the anti-trisaccharide antibody, although directed to the disaccharide, did not exactly duplicate the reactivity pattern of the anti-disaccharide.