Synthesis of sequences 1–16 and 63–95 of Cerebratulus lacteus toxin AIII: Hemolytic activity in a toxin fragment

A. BALASUBRAMANIAM; R. F. MURPHY; K. M. BLUMENTHAL

doi:10.1111/j.1399-3011.1986.tb01049.x

Synthesis of sequences 1–16 and 63–95 of Cerebratulus lacteus toxin AIII: Hemolytic activity in a toxin fragment

A. BALASUBRAMANIAM
, R. F. MURPHY
, K. M. BLUMENTHAL

Biochemistry

University of Cincinnati

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Sequences 1–16 and 63–95 of C. lacteus toxin AIII were synthesized on benz‐hydrylamine and PAM resins respectively; the crude peptide products released by HF contained 90 and 33% of the target peptides. Fragment 63–95 was nearly a full agonist, producing > 90% of the hemolytic activity of the intact toxin molecule at 10³‐fold higher concentrations. Fragment 1–16 had no activity.

Original language	English
Pages (from-to)	508-513
Number of pages	6
Journal	International Journal of Peptide and Protein Research
Volume	27
Issue number	5
DOIs	https://doi.org/10.1111/j.1399-3011.1986.tb01049.x
State	Published - May 1986

Keywords

cytolysis
high pressure liquid chromatography
solid phase synthesis
toxin AIII

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10.1111/j.1399-3011.1986.tb01049.x

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title = "Synthesis of sequences 1{\textendash}16 and 63{\textendash}95 of Cerebratulus lacteus toxin AIII: Hemolytic activity in a toxin fragment",

abstract = "Sequences 1{\textendash}16 and 63{\textendash}95 of C. lacteus toxin AIII were synthesized on benz‐hydrylamine and PAM resins respectively; the crude peptide products released by HF contained 90 and 33\% of the target peptides. Fragment 63{\textendash}95 was nearly a full agonist, producing > 90\% of the hemolytic activity of the intact toxin molecule at 103‐fold higher concentrations. Fragment 1{\textendash}16 had no activity.",

keywords = "cytolysis, high pressure liquid chromatography, solid phase synthesis, toxin AIII",

author = "A. BALASUBRAMANIAM and MURPHY, \{R. F.\} and BLUMENTHAL, \{K. M.\}",

year = "1986",

month = may,

doi = "10.1111/j.1399-3011.1986.tb01049.x",

language = "English",

volume = "27",

pages = "508--513",

journal = "International Journal of Peptide and Protein Research",

issn = "0367-8377",

publisher = "Wiley-Blackwell Publishing Ltd",

number = "5",

}

TY - JOUR

T1 - Synthesis of sequences 1–16 and 63–95 of Cerebratulus lacteus toxin AIII

T2 - Hemolytic activity in a toxin fragment

AU - BALASUBRAMANIAM, A.

AU - MURPHY, R. F.

AU - BLUMENTHAL, K. M.

PY - 1986/5

Y1 - 1986/5

N2 - Sequences 1–16 and 63–95 of C. lacteus toxin AIII were synthesized on benz‐hydrylamine and PAM resins respectively; the crude peptide products released by HF contained 90 and 33% of the target peptides. Fragment 63–95 was nearly a full agonist, producing > 90% of the hemolytic activity of the intact toxin molecule at 103‐fold higher concentrations. Fragment 1–16 had no activity.

AB - Sequences 1–16 and 63–95 of C. lacteus toxin AIII were synthesized on benz‐hydrylamine and PAM resins respectively; the crude peptide products released by HF contained 90 and 33% of the target peptides. Fragment 63–95 was nearly a full agonist, producing > 90% of the hemolytic activity of the intact toxin molecule at 103‐fold higher concentrations. Fragment 1–16 had no activity.

KW - cytolysis

KW - high pressure liquid chromatography

KW - solid phase synthesis

KW - toxin AIII

UR - https://www.scopus.com/pages/publications/0022709929

U2 - 10.1111/j.1399-3011.1986.tb01049.x

DO - 10.1111/j.1399-3011.1986.tb01049.x

M3 - Article

C2 - 2874118

AN - SCOPUS:0022709929

SN - 0367-8377

VL - 27

SP - 508

EP - 513

JO - International Journal of Peptide and Protein Research

JF - International Journal of Peptide and Protein Research

IS - 5

ER -

Synthesis of sequences 1–16 and 63–95 of Cerebratulus lacteus toxin AIII: Hemolytic activity in a toxin fragment

Abstract

Keywords

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