Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing

Lianjiang Tan; Yazhi Liu; Xiaowei Li; Xin Yan Wu; Bing Gong; Yu Mei Shen; Zhifeng Shao

doi:10.1039/c5cc09578f

Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing

Lianjiang Tan
, Yazhi Liu
, Xiaowei Li
, Xin Yan Wu
, Bing Gong
, Yu Mei Shen
, Zhifeng Shao

Chemistry

Shanghai Jiao Tong University
East China University of Science and Technology

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

An acid-cleavable linker based on a dimethylketal moiety was synthesized and used to connect a nucleotide with a fluorophore to produce a 3′-OH unblocked nucleotide analogue as an excellent reversible terminator for DNA sequencing by synthesis.

Original language	English
Pages (from-to)	2549-2552
Number of pages	4
Journal	Chemical Communications
Volume	52
Issue number	12
DOIs	https://doi.org/10.1039/c5cc09578f
State	Published - 2016

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title = "Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing",

abstract = "An acid-cleavable linker based on a dimethylketal moiety was synthesized and used to connect a nucleotide with a fluorophore to produce a 3′-OH unblocked nucleotide analogue as an excellent reversible terminator for DNA sequencing by synthesis.",

author = "Lianjiang Tan and Yazhi Liu and Xiaowei Li and Wu, \{Xin Yan\} and Bing Gong and Shen, \{Yu Mei\} and Zhifeng Shao",

note = "Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2016.",

year = "2016",

doi = "10.1039/c5cc09578f",

language = "English",

volume = "52",

pages = "2549--2552",

journal = "Chemical Communications",

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TY - JOUR

T1 - Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing

AU - Tan, Lianjiang

AU - Liu, Yazhi

AU - Li, Xiaowei

AU - Wu, Xin Yan

AU - Gong, Bing

AU - Shen, Yu Mei

AU - Shao, Zhifeng

N1 - Publisher Copyright: © The Royal Society of Chemistry 2016.

PY - 2016

Y1 - 2016

N2 - An acid-cleavable linker based on a dimethylketal moiety was synthesized and used to connect a nucleotide with a fluorophore to produce a 3′-OH unblocked nucleotide analogue as an excellent reversible terminator for DNA sequencing by synthesis.

AB - An acid-cleavable linker based on a dimethylketal moiety was synthesized and used to connect a nucleotide with a fluorophore to produce a 3′-OH unblocked nucleotide analogue as an excellent reversible terminator for DNA sequencing by synthesis.

UR - https://www.scopus.com/pages/publications/84958291814

U2 - 10.1039/c5cc09578f

DO - 10.1039/c5cc09578f

M3 - Article

C2 - 26744748

AN - SCOPUS:84958291814

SN - 1359-7345

VL - 52

SP - 2549

EP - 2552

JO - Chemical Communications

JF - Chemical Communications

IS - 12

ER -

Synthesis and evaluations of an acid-cleavable, fluorescently labeled nucleotide as a reversible terminator for DNA sequencing

Abstract

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