Substitution of a non-retinal phospholipase C in Drosophila phototransduction

S. Kim; D. M. Chen; K. Zavarella; C. F. Fourtner; W. S. Stark; Randall D. Shortridge

doi:10.1046/j.1365-2583.2003.00396.x

Substitution of a non-retinal phospholipase C in Drosophila phototransduction

S. Kim
, D. M. Chen
, K. Zavarella
, C. F. Fourtner
, W. S. Stark
, Randall D. Shortridge

Biological Sciences

SUNY Buffalo
Novartis
Saint Louis University
Indiana University Bloomington
IDna Inc.

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The Drosophila norpA gene encodes at least two subtypes of phospholipase C (PLC), one of which is essential for phototransduction and the other is utilized in signalling pathways other than phototransduction. The two subtypes of norpA-PLC differ by 14 amino acids that have been proposed as important for the function of PLC in different signalling pathways. The present study aimed to determine whether norpA subtype II enzyme can functionally substitute for the subtype I enzyme in the phototransduction pathway. We found that the non-retinal norpA-PLC enzyme can substitute for its retinal counterpart, but that there is a reduced rate of repolarization of photoreceptors following intense light stimuli. This reduced repolarization might be due to the inability of a regulatory component being able to interact with the non-retinal norpA-PLC enzyme.

Original language	English
Pages (from-to)	147-153
Number of pages	7
Journal	Insect Molecular Biology
Volume	12
Issue number	2
DOIs	https://doi.org/10.1046/j.1365-2583.2003.00396.x
State	Published - Apr 2003

Keywords

Phospholipase-C
Phototransduction
Repolarization
Retina
Vision

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10.1046/j.1365-2583.2003.00396.x

Cite this

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title = "Substitution of a non-retinal phospholipase C in Drosophila phototransduction",

abstract = "The Drosophila norpA gene encodes at least two subtypes of phospholipase C (PLC), one of which is essential for phototransduction and the other is utilized in signalling pathways other than phototransduction. The two subtypes of norpA-PLC differ by 14 amino acids that have been proposed as important for the function of PLC in different signalling pathways. The present study aimed to determine whether norpA subtype II enzyme can functionally substitute for the subtype I enzyme in the phototransduction pathway. We found that the non-retinal norpA-PLC enzyme can substitute for its retinal counterpart, but that there is a reduced rate of repolarization of photoreceptors following intense light stimuli. This reduced repolarization might be due to the inability of a regulatory component being able to interact with the non-retinal norpA-PLC enzyme.",

keywords = "Phospholipase-C, Phototransduction, Repolarization, Retina, Vision",

author = "S. Kim and Chen, \{D. M.\} and K. Zavarella and Fourtner, \{C. F.\} and Stark, \{W. S.\} and Shortridge, \{Randall D.\}",

year = "2003",

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doi = "10.1046/j.1365-2583.2003.00396.x",

language = "English",

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TY - JOUR

T1 - Substitution of a non-retinal phospholipase C in Drosophila phototransduction

AU - Kim, S.

AU - Chen, D. M.

AU - Zavarella, K.

AU - Fourtner, C. F.

AU - Stark, W. S.

AU - Shortridge, Randall D.

PY - 2003/4

Y1 - 2003/4

N2 - The Drosophila norpA gene encodes at least two subtypes of phospholipase C (PLC), one of which is essential for phototransduction and the other is utilized in signalling pathways other than phototransduction. The two subtypes of norpA-PLC differ by 14 amino acids that have been proposed as important for the function of PLC in different signalling pathways. The present study aimed to determine whether norpA subtype II enzyme can functionally substitute for the subtype I enzyme in the phototransduction pathway. We found that the non-retinal norpA-PLC enzyme can substitute for its retinal counterpart, but that there is a reduced rate of repolarization of photoreceptors following intense light stimuli. This reduced repolarization might be due to the inability of a regulatory component being able to interact with the non-retinal norpA-PLC enzyme.

AB - The Drosophila norpA gene encodes at least two subtypes of phospholipase C (PLC), one of which is essential for phototransduction and the other is utilized in signalling pathways other than phototransduction. The two subtypes of norpA-PLC differ by 14 amino acids that have been proposed as important for the function of PLC in different signalling pathways. The present study aimed to determine whether norpA subtype II enzyme can functionally substitute for the subtype I enzyme in the phototransduction pathway. We found that the non-retinal norpA-PLC enzyme can substitute for its retinal counterpart, but that there is a reduced rate of repolarization of photoreceptors following intense light stimuli. This reduced repolarization might be due to the inability of a regulatory component being able to interact with the non-retinal norpA-PLC enzyme.

KW - Phospholipase-C

KW - Phototransduction

KW - Repolarization

KW - Retina

KW - Vision

UR - https://www.scopus.com/pages/publications/0242417113

U2 - 10.1046/j.1365-2583.2003.00396.x

DO - 10.1046/j.1365-2583.2003.00396.x

M3 - Article

C2 - 12653936

AN - SCOPUS:0242417113

SN - 0962-1075

VL - 12

SP - 147

EP - 153

JO - Insect Molecular Biology

JF - Insect Molecular Biology

IS - 2

ER -

Substitution of a non-retinal phospholipase C in Drosophila phototransduction

Abstract

Keywords

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