Styryl dyes as two-photon excited fluorescent probes for DNA detection and two-photon laser scanning fluorescence microscopy of living cells

Valentyna P. Tokar; Mykhaylo Yu Losytskyy; Tymish Y. Ohulchanskyy; Dmytro V. Kryvorotenko; Vladyslava B. Kovalska; Anatoliy O. Balanda; Igor M. Dmytruk; Vadym M. Prokopets; Sergiy M. Yarmoluk; Valeriy M. Yashchuk

doi:10.1007/s10895-010-0630-4

Styryl dyes as two-photon excited fluorescent probes for DNA detection and two-photon laser scanning fluorescence microscopy of living cells

Valentyna P. Tokar
, Mykhaylo Yu Losytskyy
, Tymish Y. Ohulchanskyy
, Dmytro V. Kryvorotenko
, Vladyslava B. Kovalska
, Anatoliy O. Balanda
, Igor M. Dmytruk
, Vadym M. Prokopets
, Sergiy M. Yarmoluk
, Valeriy M. Yashchuk

Department of Chemical and Biological Engineering

NASU - Institute of Molecular Biology and Genetics
Kyiv National Taras Shevchenko University

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Spectral-fluorescent properties of benzothiazole styryl monomer (Bos-3) and homodimer (DBos-21) dyes in presence of DNA were studied. The dyes enhance their fluorescence intensity in 2-3 orders of magnitude upon interaction with DNA. Studied styrylcyanines in DNA presence demonstrate rather high values of two-photon absorption (TPA) cross-section, which are comparable with the values of TPA cross section of the rhodamine dyes. An applicability of the styrylcyanines as probes for the fluorescence microscopy of living cells was studied. It was shown that both dyes are cell-permeable but homodimer dye DBos-21 produces noticeably brighter staining of HeLa cells comparing with monomer dye Bos-3. Molecules of DBos-21 initially bind to the nucleic acids- containing cell organelles (presumable mitochondria) and are able to penetrate into the cell nucleus. Thus, homodimer styryl DBos-21 dye is viewed as efficient stain for single-photon and two-photon excitation fluorescence imaging of living cells.

Original language	English
Pages (from-to)	865-872
Number of pages	8
Journal	Journal of Fluorescence
Volume	20
Issue number	4
DOIs	https://doi.org/10.1007/s10895-010-0630-4
State	Published - Jul 2010

Keywords

DNA detection
Fluorescence microscopy
Fluorescent probes
Two-photon excitation

Access to Document

10.1007/s10895-010-0630-4

Cite this

Tokar, V. P., Losytskyy, M. Y., Ohulchanskyy, T. Y., Kryvorotenko, D. V., Kovalska, V. B., Balanda, A. O., Dmytruk, I. M., Prokopets, V. M., Yarmoluk, S. M., & Yashchuk, V. M. (2010). Styryl dyes as two-photon excited fluorescent probes for DNA detection and two-photon laser scanning fluorescence microscopy of living cells. Journal of Fluorescence, 20(4), 865-872. https://doi.org/10.1007/s10895-010-0630-4

@article{e5059579985642b2817416243d3471da,

title = "Styryl dyes as two-photon excited fluorescent probes for DNA detection and two-photon laser scanning fluorescence microscopy of living cells",

abstract = "Spectral-fluorescent properties of benzothiazole styryl monomer (Bos-3) and homodimer (DBos-21) dyes in presence of DNA were studied. The dyes enhance their fluorescence intensity in 2-3 orders of magnitude upon interaction with DNA. Studied styrylcyanines in DNA presence demonstrate rather high values of two-photon absorption (TPA) cross-section, which are comparable with the values of TPA cross section of the rhodamine dyes. An applicability of the styrylcyanines as probes for the fluorescence microscopy of living cells was studied. It was shown that both dyes are cell-permeable but homodimer dye DBos-21 produces noticeably brighter staining of HeLa cells comparing with monomer dye Bos-3. Molecules of DBos-21 initially bind to the nucleic acids- containing cell organelles (presumable mitochondria) and are able to penetrate into the cell nucleus. Thus, homodimer styryl DBos-21 dye is viewed as efficient stain for single-photon and two-photon excitation fluorescence imaging of living cells.",

keywords = "DNA detection, Fluorescence microscopy, Fluorescent probes, Two-photon excitation",

author = "Tokar, \{Valentyna P.\} and Losytskyy, \{Mykhaylo Yu\} and Ohulchanskyy, \{Tymish Y.\} and Kryvorotenko, \{Dmytro V.\} and Kovalska, \{Vladyslava B.\} and Balanda, \{Anatoliy O.\} and Dmytruk, \{Igor M.\} and Prokopets, \{Vadym M.\} and Yarmoluk, \{Sergiy M.\} and Yashchuk, \{Valeriy M.\}",

year = "2010",

month = jul,

doi = "10.1007/s10895-010-0630-4",

language = "English",

volume = "20",

pages = "865--872",

journal = "Journal of Fluorescence",

issn = "1053-0509",

publisher = "Springer",

number = "4",

}

Tokar, VP, Losytskyy, MY, Ohulchanskyy, TY, Kryvorotenko, DV, Kovalska, VB, Balanda, AO, Dmytruk, IM, Prokopets, VM, Yarmoluk, SM & Yashchuk, VM 2010, 'Styryl dyes as two-photon excited fluorescent probes for DNA detection and two-photon laser scanning fluorescence microscopy of living cells', Journal of Fluorescence, vol. 20, no. 4, pp. 865-872. https://doi.org/10.1007/s10895-010-0630-4

TY - JOUR

T1 - Styryl dyes as two-photon excited fluorescent probes for DNA detection and two-photon laser scanning fluorescence microscopy of living cells

AU - Tokar, Valentyna P.

AU - Losytskyy, Mykhaylo Yu

AU - Ohulchanskyy, Tymish Y.

AU - Kryvorotenko, Dmytro V.

AU - Kovalska, Vladyslava B.

AU - Balanda, Anatoliy O.

AU - Dmytruk, Igor M.

AU - Prokopets, Vadym M.

AU - Yarmoluk, Sergiy M.

AU - Yashchuk, Valeriy M.

PY - 2010/7

Y1 - 2010/7

N2 - Spectral-fluorescent properties of benzothiazole styryl monomer (Bos-3) and homodimer (DBos-21) dyes in presence of DNA were studied. The dyes enhance their fluorescence intensity in 2-3 orders of magnitude upon interaction with DNA. Studied styrylcyanines in DNA presence demonstrate rather high values of two-photon absorption (TPA) cross-section, which are comparable with the values of TPA cross section of the rhodamine dyes. An applicability of the styrylcyanines as probes for the fluorescence microscopy of living cells was studied. It was shown that both dyes are cell-permeable but homodimer dye DBos-21 produces noticeably brighter staining of HeLa cells comparing with monomer dye Bos-3. Molecules of DBos-21 initially bind to the nucleic acids- containing cell organelles (presumable mitochondria) and are able to penetrate into the cell nucleus. Thus, homodimer styryl DBos-21 dye is viewed as efficient stain for single-photon and two-photon excitation fluorescence imaging of living cells.

AB - Spectral-fluorescent properties of benzothiazole styryl monomer (Bos-3) and homodimer (DBos-21) dyes in presence of DNA were studied. The dyes enhance their fluorescence intensity in 2-3 orders of magnitude upon interaction with DNA. Studied styrylcyanines in DNA presence demonstrate rather high values of two-photon absorption (TPA) cross-section, which are comparable with the values of TPA cross section of the rhodamine dyes. An applicability of the styrylcyanines as probes for the fluorescence microscopy of living cells was studied. It was shown that both dyes are cell-permeable but homodimer dye DBos-21 produces noticeably brighter staining of HeLa cells comparing with monomer dye Bos-3. Molecules of DBos-21 initially bind to the nucleic acids- containing cell organelles (presumable mitochondria) and are able to penetrate into the cell nucleus. Thus, homodimer styryl DBos-21 dye is viewed as efficient stain for single-photon and two-photon excitation fluorescence imaging of living cells.

KW - DNA detection

KW - Fluorescence microscopy

KW - Fluorescent probes

KW - Two-photon excitation

UR - https://www.scopus.com/pages/publications/77955658819

U2 - 10.1007/s10895-010-0630-4

DO - 10.1007/s10895-010-0630-4

M3 - Article

C2 - 20198411

AN - SCOPUS:77955658819

SN - 1053-0509

VL - 20

SP - 865

EP - 872

JO - Journal of Fluorescence

JF - Journal of Fluorescence

IS - 4

ER -

Styryl dyes as two-photon excited fluorescent probes for DNA detection and two-photon laser scanning fluorescence microscopy of living cells

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this