Studies on the mechanism of action of a targeted chemotherapeutic drug in living cancer cells by two photon laser scanning microspectrofluorometry

Xiaopeng Wang; Haridas E. Pudavar; Rakesh Kapoor; Linda J. Krebs; Earl J. Bergey; Charles Liebow; Paras N. Prasad; Attila Nagy; Andrew V. Schally

doi:10.1117/1.1385511

Studies on the mechanism of action of a targeted chemotherapeutic drug in living cancer cells by two photon laser scanning microspectrofluorometry

Xiaopeng Wang
, Haridas E. Pudavar
, Rakesh Kapoor
, Linda J. Krebs
, Earl J. Bergey
, Charles Liebow
, Paras N. Prasad
, Attila Nagy
, Andrew V. Schally

Chemistry

SUNY Buffalo
Tulane University

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

In this study, we present a spectroscopic study of the entry pattern of a chemotherapeutic drug (AN-152) and its carrier hormone ([D-Lys⁶]LH-RH) into living cancer cells, with the help of our two-photon probes and a home-built localized microspectrofluorometer coupled with two photon laser scanning microscope (TPLSM). Due to the inherent localization ability of TPLSM, we were able to identify the drug and carrier location in different compartments of the cancer cells in vitro. The apparent doxorubicin-assisted nucleic accumulation of AN-152 suggests a possible nuclear action of the drug on cell proliferation.

Original language	English
Pages (from-to)	319-325
Number of pages	7
Journal	Journal of Biomedical Optics
Volume	6
Issue number	3
DOIs	https://doi.org/10.1117/1.1385511
State	Published - Jul 2001

Keywords

Doxorubicin
Drug trafficking
LH-RH
Multicolor imaging
TPLSMF
Two-photon laser scanning microfluorometry

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10.1117/1.1385511

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title = "Studies on the mechanism of action of a targeted chemotherapeutic drug in living cancer cells by two photon laser scanning microspectrofluorometry",

abstract = "In this study, we present a spectroscopic study of the entry pattern of a chemotherapeutic drug (AN-152) and its carrier hormone ([D-Lys6]LH-RH) into living cancer cells, with the help of our two-photon probes and a home-built localized microspectrofluorometer coupled with two photon laser scanning microscope (TPLSM). Due to the inherent localization ability of TPLSM, we were able to identify the drug and carrier location in different compartments of the cancer cells in vitro. The apparent doxorubicin-assisted nucleic accumulation of AN-152 suggests a possible nuclear action of the drug on cell proliferation.",

keywords = "Doxorubicin, Drug trafficking, LH-RH, Multicolor imaging, TPLSMF, Two-photon laser scanning microfluorometry",

author = "Xiaopeng Wang and Pudavar, \{Haridas E.\} and Rakesh Kapoor and Krebs, \{Linda J.\} and Bergey, \{Earl J.\} and Charles Liebow and Prasad, \{Paras N.\} and Attila Nagy and Schally, \{Andrew V.\}",

year = "2001",

month = jul,

doi = "10.1117/1.1385511",

language = "English",

volume = "6",

pages = "319--325",

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T1 - Studies on the mechanism of action of a targeted chemotherapeutic drug in living cancer cells by two photon laser scanning microspectrofluorometry

AU - Wang, Xiaopeng

AU - Pudavar, Haridas E.

AU - Kapoor, Rakesh

AU - Krebs, Linda J.

AU - Bergey, Earl J.

AU - Liebow, Charles

AU - Prasad, Paras N.

AU - Nagy, Attila

AU - Schally, Andrew V.

PY - 2001/7

Y1 - 2001/7

N2 - In this study, we present a spectroscopic study of the entry pattern of a chemotherapeutic drug (AN-152) and its carrier hormone ([D-Lys6]LH-RH) into living cancer cells, with the help of our two-photon probes and a home-built localized microspectrofluorometer coupled with two photon laser scanning microscope (TPLSM). Due to the inherent localization ability of TPLSM, we were able to identify the drug and carrier location in different compartments of the cancer cells in vitro. The apparent doxorubicin-assisted nucleic accumulation of AN-152 suggests a possible nuclear action of the drug on cell proliferation.

AB - In this study, we present a spectroscopic study of the entry pattern of a chemotherapeutic drug (AN-152) and its carrier hormone ([D-Lys6]LH-RH) into living cancer cells, with the help of our two-photon probes and a home-built localized microspectrofluorometer coupled with two photon laser scanning microscope (TPLSM). Due to the inherent localization ability of TPLSM, we were able to identify the drug and carrier location in different compartments of the cancer cells in vitro. The apparent doxorubicin-assisted nucleic accumulation of AN-152 suggests a possible nuclear action of the drug on cell proliferation.

KW - Doxorubicin

KW - Drug trafficking

KW - LH-RH

KW - Multicolor imaging

KW - TPLSMF

KW - Two-photon laser scanning microfluorometry

UR - https://www.scopus.com/pages/publications/0035384695

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DO - 10.1117/1.1385511

M3 - Article

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AN - SCOPUS:0035384695

SN - 1083-3668

VL - 6

SP - 319

EP - 325

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

IS - 3

ER -

Studies on the mechanism of action of a targeted chemotherapeutic drug in living cancer cells by two photon laser scanning microspectrofluorometry

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Keywords

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