TY - GEN
T1 - Studies of transport limitations in the dextran layer of the BIACORE optical biosensor
AU - Sikavitsas, Vassilios I.
AU - Nitsche, Johannes M.
AU - Mountziaris, T. J.
AU - Daiss, John L.
PY - 1999
Y1 - 1999
N2 - Surface plasmon resonance based biosensors have become increasingly popular for the measurement of the kinetic constants in a wide variety of biomolecular interactions. One of the most popular biosensors based on this principle is the BIACORE instrument. A mathematical model is presented describing biomolecular interactions in the flowcell of the BIACORE. The model accounts not only for mass transport limitations due to the concentration gradient directly above the sensor's dextran hydrogel matrix, but also for the effects of hindered transport of the mobile reactants into the dextran hydrogel matrix while diffusing towards their immobilized counterparts. Experiments have been also conducted on a BIACORE 2000 instrument that validate the proposed model.
AB - Surface plasmon resonance based biosensors have become increasingly popular for the measurement of the kinetic constants in a wide variety of biomolecular interactions. One of the most popular biosensors based on this principle is the BIACORE instrument. A mathematical model is presented describing biomolecular interactions in the flowcell of the BIACORE. The model accounts not only for mass transport limitations due to the concentration gradient directly above the sensor's dextran hydrogel matrix, but also for the effects of hindered transport of the mobile reactants into the dextran hydrogel matrix while diffusing towards their immobilized counterparts. Experiments have been also conducted on a BIACORE 2000 instrument that validate the proposed model.
UR - https://www.scopus.com/pages/publications/0033350928
M3 - Conference contribution
AN - SCOPUS:0033350928
SN - 0780356756
T3 - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
SP - 808
BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PB - IEEE
T2 - Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
Y2 - 13 October 1999 through 16 October 1999
ER -