Structural genomics of pathogenic protozoa: an overview.

Erkang Fan; David Baker; Stanley Fields; Michael H. Gelb; Frederick S. Buckner; Wesley C. Van Voorhis; Eric Phizicky; Mark Dumont; Christopher Mehlin; Elizabeth Grayhack; Mark Sullivan; Christophe Verlinde; George Detitta; Deirdre R. Meldrum; Ethan A. Merritt; Thomas Earnest; Michael Soltis; Frank Zucker; Peter J. Myler; Lori Schoenfeld; David Kim; Liz Worthey; Doug Lacount; Marissa Vignali; Jizhen Li; Somnath Mondal; Archna Massey; Brian Carroll; Stacey Gulde; Joseph Luft; Larry Desoto; Mark Holl; Jonathan Caruthers; Jürgen Bosch; Mark Robien; Tracy Arakaki; Margaret Holmes; Isolde Le Trong; Wim G.J. Hol

doi:10.1007/978-1-60327-058-8_33

Structural genomics of pathogenic protozoa: an overview.

Erkang Fan
, David Baker
, Stanley Fields
, Michael H. Gelb
, Frederick S. Buckner
, Wesley C. Van Voorhis
, Eric Phizicky
, Mark Dumont
, Christopher Mehlin
, Elizabeth Grayhack
, Mark Sullivan
, Christophe Verlinde
, George Detitta
, Deirdre R. Meldrum
, Ethan A. Merritt
, Thomas Earnest
, Michael Soltis
, Frank Zucker
, Peter J. Myler
, Lori Schoenfeld

David Kim, Liz Worthey, Doug Lacount, Marissa Vignali, Jizhen Li, Somnath Mondal, Archna Massey, Brian Carroll, Stacey Gulde, Joseph Luft, Larry Desoto, Mark Holl, Jonathan Caruthers, Jürgen Bosch, Mark Robien, Tracy Arakaki, Margaret Holmes, Isolde Le Trong, Wim G.J. Hol

Department of Structural Biology

University of Washington

Research output: Contribution to journal › Review article › peer-review

41 Scopus citations

Abstract

The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.

Original language	English
Pages (from-to)	497-513
Number of pages	17
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	426
DOIs	https://doi.org/10.1007/978-1-60327-058-8_33
State	Published - 2008

Access to Document

10.1007/978-1-60327-058-8_33

Cite this

Fan, E., Baker, D., Fields, S., Gelb, M. H., Buckner, F. S., Van Voorhis, W. C., Phizicky, E., Dumont, M., Mehlin, C., Grayhack, E., Sullivan, M., Verlinde, C., Detitta, G., Meldrum, D. R., Merritt, E. A., Earnest, T., Soltis, M., Zucker, F., Myler, P. J., ... Hol, W. G. J. (2008). Structural genomics of pathogenic protozoa: an overview. Methods in molecular biology (Clifton, N.J.), 426, 497-513. https://doi.org/10.1007/978-1-60327-058-8_33

@article{1e37cbf98c854aa79d0976b2299bbd9c,

title = "Structural genomics of pathogenic protozoa: an overview.",

abstract = "The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of {"}co-crystallants,{"} and capillary crystallization. {"}Fragment cocktail crystallography{"} for medical structural genomics is also described.",

author = "Erkang Fan and David Baker and Stanley Fields and Gelb, \{Michael H.\} and Buckner, \{Frederick S.\} and \{Van Voorhis\}, \{Wesley C.\} and Eric Phizicky and Mark Dumont and Christopher Mehlin and Elizabeth Grayhack and Mark Sullivan and Christophe Verlinde and George Detitta and Meldrum, \{Deirdre R.\} and Merritt, \{Ethan A.\} and Thomas Earnest and Michael Soltis and Frank Zucker and Myler, \{Peter J.\} and Lori Schoenfeld and David Kim and Liz Worthey and Doug Lacount and Marissa Vignali and Jizhen Li and Somnath Mondal and Archna Massey and Brian Carroll and Stacey Gulde and Joseph Luft and Larry Desoto and Mark Holl and Jonathan Caruthers and J{\"u}rgen Bosch and Mark Robien and Tracy Arakaki and Margaret Holmes and \{Le Trong\}, Isolde and Hol, \{Wim G.J.\}",

year = "2008",

doi = "10.1007/978-1-60327-058-8\_33",

language = "English",

volume = "426",

pages = "497--513",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

publisher = "Humana Press Inc.",

}

Fan, E, Baker, D, Fields, S, Gelb, MH, Buckner, FS, Van Voorhis, WC, Phizicky, E, Dumont, M, Mehlin, C, Grayhack, E, Sullivan, M, Verlinde, C, Detitta, G, Meldrum, DR, Merritt, EA, Earnest, T, Soltis, M, Zucker, F, Myler, PJ, Schoenfeld, L, Kim, D, Worthey, L, Lacount, D, Vignali, M, Li, J, Mondal, S, Massey, A, Carroll, B, Gulde, S, Luft, J, Desoto, L, Holl, M, Caruthers, J, Bosch, J, Robien, M, Arakaki, T, Holmes, M, Le Trong, I & Hol, WGJ 2008, 'Structural genomics of pathogenic protozoa: an overview.', Methods in molecular biology (Clifton, N.J.), vol. 426, pp. 497-513. https://doi.org/10.1007/978-1-60327-058-8_33

TY - JOUR

T1 - Structural genomics of pathogenic protozoa

T2 - an overview.

AU - Fan, Erkang

AU - Baker, David

AU - Fields, Stanley

AU - Gelb, Michael H.

AU - Buckner, Frederick S.

AU - Van Voorhis, Wesley C.

AU - Phizicky, Eric

AU - Dumont, Mark

AU - Mehlin, Christopher

AU - Grayhack, Elizabeth

AU - Sullivan, Mark

AU - Verlinde, Christophe

AU - Detitta, George

AU - Meldrum, Deirdre R.

AU - Merritt, Ethan A.

AU - Earnest, Thomas

AU - Soltis, Michael

AU - Zucker, Frank

AU - Myler, Peter J.

AU - Schoenfeld, Lori

AU - Kim, David

AU - Worthey, Liz

AU - Lacount, Doug

AU - Vignali, Marissa

AU - Li, Jizhen

AU - Mondal, Somnath

AU - Massey, Archna

AU - Carroll, Brian

AU - Gulde, Stacey

AU - Luft, Joseph

AU - Desoto, Larry

AU - Holl, Mark

AU - Caruthers, Jonathan

AU - Bosch, Jürgen

AU - Robien, Mark

AU - Arakaki, Tracy

AU - Holmes, Margaret

AU - Le Trong, Isolde

AU - Hol, Wim G.J.

PY - 2008

Y1 - 2008

N2 - The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.

AB - The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.

UR - https://www.scopus.com/pages/publications/47349086162

U2 - 10.1007/978-1-60327-058-8_33

DO - 10.1007/978-1-60327-058-8_33

M3 - Review article

C2 - 18542886

AN - SCOPUS:47349086162

SN - 1064-3745

VL - 426

SP - 497

EP - 513

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -

Structural genomics of pathogenic protozoa: an overview.

Abstract

Access to Document

Other files and links

Fingerprint

Cite this