Stress resistant human embryonic stem cells as a potential source for the identification of novel cancer stem cell markers

Shaker A. Mousa; Thangirala Sudha; Evgeny Dyskin; Usawadee Dier; Christine Gallati; Christine Hanko; Sridar V. Chittur; Abdelhadi Rebbaa

doi:10.1016/j.canlet.2009.08.018

Stress resistant human embryonic stem cells as a potential source for the identification of novel cancer stem cell markers

Shaker A. Mousa
, Thangirala Sudha
, Evgeny Dyskin
, Usawadee Dier
, Christine Gallati
, Christine Hanko
, Sridar V. Chittur
, Abdelhadi Rebbaa

Orthopaedics

Albany College of Pharmacy
SUNY Albany
University of Pittsburgh

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Cancer stem cells are known for their inherent resistance to therapy. Here we investigated whether normal stem cells with acquired resistance to stress can be used to identify novel markers of cancer stem cells. For this, we generated a human embryonic stem cell line resistant to Trichostatin A and analyzed changes in its gene expression. The resistant cells over-expressed various genes associated with tumor aggressiveness, many of which are also expressed in the CD133+ glioma cancer stem cells. These findings suggest that stress-resistant stem cells generated in vitro may be useful for the discovery of novel markers of cancer stem cells.

Original language	English
Pages (from-to)	208-216
Number of pages	9
Journal	Cancer Letters
Volume	289
Issue number	2
DOIs	https://doi.org/10.1016/j.canlet.2009.08.018
State	Published - Mar 28 2010

Keywords

Cancer stem cells
cDNA array
Drug resistance
Markers
Trichostatin A

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10.1016/j.canlet.2009.08.018

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title = "Stress resistant human embryonic stem cells as a potential source for the identification of novel cancer stem cell markers",

abstract = "Cancer stem cells are known for their inherent resistance to therapy. Here we investigated whether normal stem cells with acquired resistance to stress can be used to identify novel markers of cancer stem cells. For this, we generated a human embryonic stem cell line resistant to Trichostatin A and analyzed changes in its gene expression. The resistant cells over-expressed various genes associated with tumor aggressiveness, many of which are also expressed in the CD133+ glioma cancer stem cells. These findings suggest that stress-resistant stem cells generated in vitro may be useful for the discovery of novel markers of cancer stem cells.",

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T1 - Stress resistant human embryonic stem cells as a potential source for the identification of novel cancer stem cell markers

AU - Mousa, Shaker A.

AU - Sudha, Thangirala

AU - Dyskin, Evgeny

AU - Dier, Usawadee

AU - Gallati, Christine

AU - Hanko, Christine

AU - Chittur, Sridar V.

AU - Rebbaa, Abdelhadi

PY - 2010/3/28

Y1 - 2010/3/28

N2 - Cancer stem cells are known for their inherent resistance to therapy. Here we investigated whether normal stem cells with acquired resistance to stress can be used to identify novel markers of cancer stem cells. For this, we generated a human embryonic stem cell line resistant to Trichostatin A and analyzed changes in its gene expression. The resistant cells over-expressed various genes associated with tumor aggressiveness, many of which are also expressed in the CD133+ glioma cancer stem cells. These findings suggest that stress-resistant stem cells generated in vitro may be useful for the discovery of novel markers of cancer stem cells.

AB - Cancer stem cells are known for their inherent resistance to therapy. Here we investigated whether normal stem cells with acquired resistance to stress can be used to identify novel markers of cancer stem cells. For this, we generated a human embryonic stem cell line resistant to Trichostatin A and analyzed changes in its gene expression. The resistant cells over-expressed various genes associated with tumor aggressiveness, many of which are also expressed in the CD133+ glioma cancer stem cells. These findings suggest that stress-resistant stem cells generated in vitro may be useful for the discovery of novel markers of cancer stem cells.

KW - Cancer stem cells

KW - cDNA array

KW - Drug resistance

KW - Markers

KW - Trichostatin A

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SP - 208

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JO - Cancer Letters

JF - Cancer Letters

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ER -

Stress resistant human embryonic stem cells as a potential source for the identification of novel cancer stem cell markers

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Keywords

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