Standard operating procedure formetabonomics studies of blood serum and plasma samples using a ¹H-NMR micro-flow probe

A standard operating procedure (SOP) is presented for high-through put metabonomics studies using a 600 MHz NMR spectrometer equipped with a micro-flow probe that is connected to an auto-sampler. The procedure is designed to minimize random and systematic variation of NMR data collection. In addition, a protocol is described to assess the quality of the data acquired byagiven NMR spectroscopist toensure that (i) all researchers involved in the NMR data acquisition of ametabonomics research program perform equally and (ii) operator-associated variation of NMR data collection is statistically not relevant for the interpretation of results obtained from multivariate data analyses.