STAINLESS STEEL AS ELECTRODE MATERIAL FOR INTRACELLULAR PH DETERMINATION.

Potentiodynamic studies are described that have shown that, at potentials that are negative with respect to Ag/AgCl, a stainless steel electrode becomes electrochemically inactive except for hydrogen ion. The cyclic voltammogram (-1. 10 V to -0. 10 V) of this electrode in biologic solutions contains two unequally sized loops separated by a node. It has been observed that the node potential, the size of the component loops, and the current level at or below the node, change linearly with pH from 6. 0 to 8. 0 and beyond. This result raises the possibility of utilizing stainless steel as an intracellular electrode and has led to a novel approach to potentiodynamic determination of pH.