Site-specific attachment of gold nanoparticles to DNA templates

K. A. Stevenson; G. Muralidharan; L. Maya; J. C. Wells; J. Barhen; T. Thundat

doi:10.1557/proc-635-c4.2

Site-specific attachment of gold nanoparticles to DNA templates

K. A. Stevenson
, G. Muralidharan
, L. Maya
, J. C. Wells
, J. Barhen
, T. Thundat

Department of Chemical and Biological Engineering

Oak Ridge National Laboratory

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

DNA was used as a scaffold for the binding of gold nanoparticles using a standard chemical technique. A DNA template was designed with amino-modified thymines located every 3.7 nm, which would allow the attachment of the carboxylic acid functionalized gold nanoparticles. The gold particles were covalently bound to the amino groups on the DNA using standard 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) chemistry in the presence of a competitor to block excess gold binding sites. The products were analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM).

Original language	English
Pages (from-to)	C421-C424
Journal	Materials Research Society Symposium - Proceedings
Volume	635
DOIs	https://doi.org/10.1557/proc-635-c4.2
State	Published - 2001
Event	Anisotropic Nanoparticles-Synthesis, Characterization and Applications - Boston, MA, United States Duration: Nov 27 2000 → Nov 29 2000

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title = "Site-specific attachment of gold nanoparticles to DNA templates",

abstract = "DNA was used as a scaffold for the binding of gold nanoparticles using a standard chemical technique. A DNA template was designed with amino-modified thymines located every 3.7 nm, which would allow the attachment of the carboxylic acid functionalized gold nanoparticles. The gold particles were covalently bound to the amino groups on the DNA using standard 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) chemistry in the presence of a competitor to block excess gold binding sites. The products were analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM).",

author = "Stevenson, \{K. A.\} and G. Muralidharan and L. Maya and Wells, \{J. C.\} and J. Barhen and T. Thundat",

year = "2001",

doi = "10.1557/proc-635-c4.2",

language = "English",

volume = "635",

pages = "C421--C424",

journal = "Materials Research Society Symposium - Proceedings",

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note = "Anisotropic Nanoparticles-Synthesis, Characterization and Applications ; Conference date: 27-11-2000 Through 29-11-2000",

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TY - JOUR

T1 - Site-specific attachment of gold nanoparticles to DNA templates

AU - Stevenson, K. A.

AU - Muralidharan, G.

AU - Maya, L.

AU - Wells, J. C.

AU - Barhen, J.

AU - Thundat, T.

PY - 2001

Y1 - 2001

N2 - DNA was used as a scaffold for the binding of gold nanoparticles using a standard chemical technique. A DNA template was designed with amino-modified thymines located every 3.7 nm, which would allow the attachment of the carboxylic acid functionalized gold nanoparticles. The gold particles were covalently bound to the amino groups on the DNA using standard 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) chemistry in the presence of a competitor to block excess gold binding sites. The products were analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM).

AB - DNA was used as a scaffold for the binding of gold nanoparticles using a standard chemical technique. A DNA template was designed with amino-modified thymines located every 3.7 nm, which would allow the attachment of the carboxylic acid functionalized gold nanoparticles. The gold particles were covalently bound to the amino groups on the DNA using standard 1-ethyl-3- (3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) chemistry in the presence of a competitor to block excess gold binding sites. The products were analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM).

UR - https://www.scopus.com/pages/publications/0034834727

U2 - 10.1557/proc-635-c4.2

DO - 10.1557/proc-635-c4.2

M3 - Conference article

AN - SCOPUS:0034834727

SN - 0272-9172

VL - 635

SP - C421-C424

JO - Materials Research Society Symposium - Proceedings

JF - Materials Research Society Symposium - Proceedings

T2 - Anisotropic Nanoparticles-Synthesis, Characterization and Applications

Y2 - 27 November 2000 through 29 November 2000

ER -

Site-specific attachment of gold nanoparticles to DNA templates

Abstract

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