TY - GEN
T1 - Single-layer microfluidic network-based combinatorial dilution for a standard simplex-lattice combinatorial design
AU - Lee, Kangsun
AU - Kim, Choong
AU - Ahn, Byungwook
AU - Lee, Hun
AU - Panchapakesan, Rajagopal
AU - Xu, Linfeng
AU - Xu, Jing
AU - Kang, Ji Yoon
AU - Oh, Kwang W.
PY - 2011
Y1 - 2011
N2 - In this paper, we presented a straightforward strategy to generate 15 combinations with 3 samples, using single-layer microfluidic network. First, we investigated the performance of the single-layer based design by computational simulation (CFD-ACE+). The simulated output concentrations were extremely close to the expected values within absolute error < 1%. Based on the simulated design, a PDMS device was fabricated by soft-lithography and tested with fluorescent dye (sodium salt). The combinatorial mixing results for 15 combinations showed good performance with absolute error less than 4%. In addition, we have also conceptually presented two liquid handing methods (bottom-up and top-down) for high-throughput screening and assay.
AB - In this paper, we presented a straightforward strategy to generate 15 combinations with 3 samples, using single-layer microfluidic network. First, we investigated the performance of the single-layer based design by computational simulation (CFD-ACE+). The simulated output concentrations were extremely close to the expected values within absolute error < 1%. Based on the simulated design, a PDMS device was fabricated by soft-lithography and tested with fluorescent dye (sodium salt). The combinatorial mixing results for 15 combinations showed good performance with absolute error less than 4%. In addition, we have also conceptually presented two liquid handing methods (bottom-up and top-down) for high-throughput screening and assay.
KW - Combinatorial dilution
KW - High-throughput screening (HTS)
KW - Microfluidic network
KW - Simplex lattice design
UR - https://www.scopus.com/pages/publications/84883754711
M3 - Conference contribution
AN - SCOPUS:84883754711
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 939
EP - 941
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -