Abstract
A method for carrying out dose-response studies of ion channel currents in cell-attached patches has been devised. Patch pipettes are filled at the tip with a solution containing one concentration of ligand and then backfilled with another. The concentration of ligand at the membrane is described as a function of time by the equation for diffusion in a cone, allowing response vs. time data to be transformed into a dose-response curve. For Xenopus myocyte cholinergic receptors, examples of the use of this method are given for several concentration-dependent reactions including blockade by the local anesthetic QX-222, activation by acetylcholine, and modulation of current amplitude by sodium ions. Several methods of analyzing the nonstationary channel kinetics are presented, including a pseudo-stationary approach that uses interval likelihood maximization.
| Original language | English |
|---|---|
| Pages (from-to) | 660-670 |
| Number of pages | 11 |
| Journal | Biophysical Journal |
| Volume | 60 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1991 |
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