Simultaneous two-component determinations by phase-resolved fluorescence spectrometry

Three approaches to phase-resolved fluorimetric determination of mixtures of 1,4-bis(5-phenyloxazol-2-yl)benzene and its dimethyl derivatives are compared, including the use of measurements at the detector phase angles required to null the phase resolved intensity contribution of each component and the use of measurements at multiple detector phase angles which are either used in an indirect-nulling method or in simultaneous-equation approach. Best results were obtained using the latter method with eight detector phase angles, and both the indirect-nulling and simutaneous-equation methods were generally superior to the method involving measurements at the "nulling" detector phase angles.