SCRMshaw: Supervised cis-regulatory module prediction for insect genomes

As the number of sequenced insect genomes continues to grow, there is a pressing need for rapid and accurate annotation of their regulatory component. SCRMshaw is a computational tool designed to predict cis-regulatory modules (“enhancers”) in the genomes of various insect species. A key advantage of SCRMshaw is its accessibility. It requires minimal resources—just a genome sequence and training data from known Drosophila regulatory sequences, which are readily available for download. Even users with modest computational skills can run SCRMshaw on a desktop computer for basic applications, although a high-performance computing cluster is recommended for optimal results. SCRMshaw can be tailored to specific needs: users can employ a single set of training data to predict enhancers associated with a particular gene expression pattern, or utilize multiple sets to provide a first-pass regulatory annotation for a newly-sequenced genome. This protocol provides an extensive update to the previously published SCRMshaw protocol and aligns with the methods used in a recent annotation of over 30 insect regulatory genomes. It includes the most recent modifications to the SCRMshaw protocol and details an end-to-end pipeline that begins with a sequenced genome and ends with a fully-annotated regulatory genome. Relevant scripts are available via GitHub, and a living protocol that will be updated as necessary is linked to this article at protocols.io.