Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: Expression, purification and crystallization

Jasper Marc G. Bondoc; Hiten J. Gutka; Mashal M. Almutairi; Ryan Patwell; Maxwell W. Rutter; Nina M. Wolf; Ram Samudrala; Shahila Mehboob; Farahnaz Movahedzadeh

doi:10.1107/S2053230X19012652

Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: Expression, purification and crystallization

Jasper Marc G. Bondoc
, Hiten J. Gutka
, Mashal M. Almutairi
, Ryan Patwell
, Maxwell W. Rutter
, Nina M. Wolf
, Ram Samudrala
, Shahila Mehboob
, Farahnaz Movahedzadeh

Department of Biomedical Informatics

University of Illinois at Chicago
Oncobiologics Inc.
King Saud University
Baylor College of Medicine
Hollingbery and Son Hops Inc.
Neugenica LLC

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Acyl carrier proteins (ACPs) are important components in fatty-acid biosynthesis in prokaryotes. Rv0100 is predicted to be an essential ACP in Mycobacterium tuberculosis, the pathogen that is the causative agent of tuberculosis, and therefore has the potential to be a novel antituberculosis drug target. Here, the successful cloning and purification of Rv0100 using Mycobacterium smegmatis as a host is reported. Crystals of the purified protein were obtained that diffracted to a resolution of 1.9 Å. Overall, this work lays the foundation for the future pursuit of drug discovery and development against this potentially novel drug target.

Original language	English
Pages (from-to)	646-651
Number of pages	6
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	75
DOIs	https://doi.org/10.1107/S2053230X19012652
State	Published - Oct 1 2019

Keywords

acyl carrier protein
Mycobacterium smegmatis
Mycobacterium tuberculosis
Rv0100

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10.1107/S2053230X19012652

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Bondoc, J. M. G., Gutka, H. J., Almutairi, M. M., Patwell, R., Rutter, M. W., Wolf, N. M., Samudrala, R., Mehboob, S., & Movahedzadeh, F. (2019). Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: Expression, purification and crystallization. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 75, 646-651. https://doi.org/10.1107/S2053230X19012652

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title = "Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: Expression, purification and crystallization",

abstract = "Acyl carrier proteins (ACPs) are important components in fatty-acid biosynthesis in prokaryotes. Rv0100 is predicted to be an essential ACP in Mycobacterium tuberculosis, the pathogen that is the causative agent of tuberculosis, and therefore has the potential to be a novel antituberculosis drug target. Here, the successful cloning and purification of Rv0100 using Mycobacterium smegmatis as a host is reported. Crystals of the purified protein were obtained that diffracted to a resolution of 1.9 {\AA}. Overall, this work lays the foundation for the future pursuit of drug discovery and development against this potentially novel drug target.",

keywords = "acyl carrier protein, Mycobacterium smegmatis, Mycobacterium tuberculosis, Rv0100",

author = "Bondoc, \{Jasper Marc G.\} and Gutka, \{Hiten J.\} and Almutairi, \{Mashal M.\} and Ryan Patwell and Rutter, \{Maxwell W.\} and Wolf, \{Nina M.\} and Ram Samudrala and Shahila Mehboob and Farahnaz Movahedzadeh",

note = "Publisher Copyright: {\textcopyright} 2019 International Union of Crystallography.",

year = "2019",

month = oct,

day = "1",

doi = "10.1107/S2053230X19012652",

language = "English",

volume = "75",

pages = "646--651",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

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Bondoc, JMG, Gutka, HJ, Almutairi, MM, Patwell, R, Rutter, MW, Wolf, NM, Samudrala, R, Mehboob, S & Movahedzadeh, F 2019, 'Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: Expression, purification and crystallization', Acta Crystallographica Section F: Structural Biology and Crystallization Communications, vol. 75, pp. 646-651. https://doi.org/10.1107/S2053230X19012652

Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: Expression, purification and crystallization. / Bondoc, Jasper Marc G.; Gutka, Hiten J.; Almutairi, Mashal M. et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 75, 01.10.2019, p. 646-651.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis

T2 - Expression, purification and crystallization

AU - Bondoc, Jasper Marc G.

AU - Gutka, Hiten J.

AU - Almutairi, Mashal M.

AU - Patwell, Ryan

AU - Rutter, Maxwell W.

AU - Wolf, Nina M.

AU - Samudrala, Ram

AU - Mehboob, Shahila

AU - Movahedzadeh, Farahnaz

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Acyl carrier proteins (ACPs) are important components in fatty-acid biosynthesis in prokaryotes. Rv0100 is predicted to be an essential ACP in Mycobacterium tuberculosis, the pathogen that is the causative agent of tuberculosis, and therefore has the potential to be a novel antituberculosis drug target. Here, the successful cloning and purification of Rv0100 using Mycobacterium smegmatis as a host is reported. Crystals of the purified protein were obtained that diffracted to a resolution of 1.9 Å. Overall, this work lays the foundation for the future pursuit of drug discovery and development against this potentially novel drug target.

AB - Acyl carrier proteins (ACPs) are important components in fatty-acid biosynthesis in prokaryotes. Rv0100 is predicted to be an essential ACP in Mycobacterium tuberculosis, the pathogen that is the causative agent of tuberculosis, and therefore has the potential to be a novel antituberculosis drug target. Here, the successful cloning and purification of Rv0100 using Mycobacterium smegmatis as a host is reported. Crystals of the purified protein were obtained that diffracted to a resolution of 1.9 Å. Overall, this work lays the foundation for the future pursuit of drug discovery and development against this potentially novel drug target.

KW - acyl carrier protein

KW - Mycobacterium smegmatis

KW - Mycobacterium tuberculosis

KW - Rv0100

UR - https://www.scopus.com/pages/publications/85072917098

U2 - 10.1107/S2053230X19012652

DO - 10.1107/S2053230X19012652

M3 - Article

C2 - 31584013

AN - SCOPUS:85072917098

SN - 1744-3091

VL - 75

SP - 646

EP - 651

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

ER -

Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: Expression, purification and crystallization

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Keywords

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