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Ribosomal movement impeded at a pseudoknot required for frameshifting

  • SUNY Buffalo

Research output: Contribution to journalArticlepeer-review

151 Scopus citations

Abstract

Translational frameshifting sometimes occurs when ribosomes encounter a "shift" site preceding a region of unusual secondary structure, which in at least three cases is known to be a pseudoknot. We provide evidence that ribosomes have a decreased rate of movement through a pseudoknot required for frameshifting. These paused ribosomes are directly situated over the shift sequence. Ribosomal pausing appears to be necessary but not sufficient for frameshifting.

Original languageEnglish
Pages (from-to)8636-8640
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number18
StatePublished - 1992

Keywords

  • Double-stranded RNA virus
  • Saccharomyces cerevisiae virus
  • Translation
  • Yeast

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