Abstract
An ion-pair reversed-phase high-performance liquid chromatographic method is presented for the determination of adriamycin and adriamycinol concentrations in rat serum and tissues. The scheme for developing the chromatographic conditions is discussed. A good separation of adriamycinol and adriamycin was obtained by using a high sodium lauryl sulphate concentration in the mobile phase. The retention times of adriamycinol, adriamycin and daunomycin (internal standard) were less than 3 min with no interfering peaks from endogenous constituents. A protein precipitation method was used to prepare the serum and tissue samples for injection.
| Original language | English |
|---|---|
| Pages (from-to) | 483-490 |
| Number of pages | 8 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 4 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1986 |
Keywords
- adriamycin and adriamycinol.
- Ion-pair HPLC
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