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Recovery in hemiparkinsonian rats following intrastriatal implantation of activated leukocytes

  • National Institutes of Health

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Behavioral improvement has been seen in hemiparkinsonian animals after surgical lesions of the denervated caudate nucleus. This study was designed to investigate the role of inflammatory cells in injury-induced recovery. A hemiparkinsonian syndrome was induced in rats by unilateral injections of 6-hydroxydopamine into the pars compacta of the substantia nigra. Phytohemagglutinin-stimulated rat peritoneal cells, predominantly T cells and macrophages, were stereotactically implanted in the lesioned caudate-putamen, and amphetamine-induced turning was used to assess recovery. Animals receiving implants of activated peritoneal cells showed a 47% decrease in amphetamine-induced turning 8 weeks after implantation, which was not seen in control or sham-operated animals. Immunocytochemistry revealed increased tyrosine hydroxylase reactive fibers in the leukocyte-implanted striatum. We conclude that implantation of activated leukocytes promotes functional recovery in hemiparkinsonian rats.

Original languageEnglish
Pages (from-to)42-48
Number of pages7
JournalBrain Research
Volume576
Issue number1
DOIs
StatePublished - Mar 27 1992

Keywords

  • 6-Hydroxydopamine
  • Activated leukocyte
  • Central nervous system implantation
  • Fluorescent activated cell sorting
  • Hemiparkinsonian rat

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