Recombinant Protein Production Using Mammalian Expression Systems

There is a rising need for large and complex recombinant proteins in biomedical research and biotechnology applications. Sophisticated mammalian protein expression systems are gaining traction to meet this need with recent advancements in transgene delivery, transgene overexpression, and protein isolation. With careful consideration of the experimental design, mammalian expression hosts hold unprecedented power in large-scale production of recombinant proteins that are traditionally refractory to biophysical and biochemical studies. This article highlights fundamental principles governing recombinant protein production in mammalian expression hosts and guides readers on key considerations for experimental design.