Recombinant expression and partial characterization of the human formyl peptide receptor

Amitabha Lala; Ashu Sharma; Hakimuddin T. Sojar; Stephen J. Radel; Robert J. Genco; Ernesto De Nardin

doi:10.1016/0167-4889(93)90208-7

Recombinant expression and partial characterization of the human formyl peptide receptor

Amitabha Lala
, Ashu Sharma
, Hakimuddin T. Sojar
, Stephen J. Radel
, Robert J. Genco
, Ernesto De Nardin

SUNY Buffalo

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

FMLP-receptor DNA was expressed in Escherichia coli. The expressed product could specifically bind FMLP. This is the first-reported expression of a functional FMLP receptor in Escherichia coli. We confirm that receptor glycosylation is not essential for ligand binding. A deletion mutant did not bind FMLP, suggesting that the deleted portion plays a role in ligand binding.

Original language	English
Pages (from-to)	302-306
Number of pages	5
Journal	Biochimica et Biophysica Acta - Molecular Cell Research
Volume	1178
Issue number	3
DOIs	https://doi.org/10.1016/0167-4889(93)90208-7
State	Published - Sep 13 1993

Keywords

(Human)
(Neutrophil)
Formyl peptide receptor
Gene expression
Ligand binding

Access to Document

10.1016/0167-4889(93)90208-7

Cite this

@article{bbe7136fcc554e9e820e123b1673ac04,

title = "Recombinant expression and partial characterization of the human formyl peptide receptor",

abstract = "FMLP-receptor DNA was expressed in Escherichia coli. The expressed product could specifically bind FMLP. This is the first-reported expression of a functional FMLP receptor in Escherichia coli. We confirm that receptor glycosylation is not essential for ligand binding. A deletion mutant did not bind FMLP, suggesting that the deleted portion plays a role in ligand binding.",

keywords = "(Human), (Neutrophil), Formyl peptide receptor, Gene expression, Ligand binding",

author = "Amitabha Lala and Ashu Sharma and Sojar, \{Hakimuddin T.\} and Radel, \{Stephen J.\} and Genco, \{Robert J.\} and \{De Nardin\}, Ernesto",

year = "1993",

month = sep,

day = "13",

doi = "10.1016/0167-4889(93)90208-7",

language = "English",

volume = "1178",

pages = "302--306",

journal = "Biochimica et Biophysica Acta - Molecular Cell Research",

issn = "0167-4889",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Recombinant expression and partial characterization of the human formyl peptide receptor

AU - Lala, Amitabha

AU - Sharma, Ashu

AU - Sojar, Hakimuddin T.

AU - Radel, Stephen J.

AU - Genco, Robert J.

AU - De Nardin, Ernesto

PY - 1993/9/13

Y1 - 1993/9/13

N2 - FMLP-receptor DNA was expressed in Escherichia coli. The expressed product could specifically bind FMLP. This is the first-reported expression of a functional FMLP receptor in Escherichia coli. We confirm that receptor glycosylation is not essential for ligand binding. A deletion mutant did not bind FMLP, suggesting that the deleted portion plays a role in ligand binding.

AB - FMLP-receptor DNA was expressed in Escherichia coli. The expressed product could specifically bind FMLP. This is the first-reported expression of a functional FMLP receptor in Escherichia coli. We confirm that receptor glycosylation is not essential for ligand binding. A deletion mutant did not bind FMLP, suggesting that the deleted portion plays a role in ligand binding.

KW - (Human)

KW - (Neutrophil)

KW - Formyl peptide receptor

KW - Gene expression

KW - Ligand binding

UR - https://www.scopus.com/pages/publications/0027244676

U2 - 10.1016/0167-4889(93)90208-7

DO - 10.1016/0167-4889(93)90208-7

M3 - Article

C2 - 8364044

AN - SCOPUS:0027244676

SN - 0167-4889

VL - 1178

SP - 302

EP - 306

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

IS - 3

ER -

Recombinant expression and partial characterization of the human formyl peptide receptor

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this