Rapid processing of the carboxyl terminus of a trypanosome variant surface glycoprotein

J. D. Bangs; D. Hereld; J. L. Krakow; G. W. Hart; P. T. Englund

doi:10.1073/pnas.82.10.3207

Rapid processing of the carboxyl terminus of a trypanosome variant surface glycoprotein

J. D. Bangs
, D. Hereld
, J. L. Krakow
, G. W. Hart
, P. T. Englund

Microbiology and Immunology

Johns Hopkins University

Research output: Contribution to journal › Article › peer-review

157 Scopus citations

Abstract

The variant surface glycoprotein of the parasite Trypanosoma brucei contains a glycolipid of unknown structure covalently attached to its COOH terminus. We have shown, by using metabolic labeling with [³⁵S]methionine or [³H]myristic acid, precipitation with specific antibodies, and NaDodSO₄/polyacrylamide gel electrophoresis, that this glycolipid is attached to the variant surface glycoprotein polypeptide within 1 min after its translation.

Original language	English
Pages (from-to)	3207-3211
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	82
Issue number	10
DOIs	https://doi.org/10.1073/pnas.82.10.3207
State	Published - 1985

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abstract = "The variant surface glycoprotein of the parasite Trypanosoma brucei contains a glycolipid of unknown structure covalently attached to its COOH terminus. We have shown, by using metabolic labeling with [35S]methionine or [3H]myristic acid, precipitation with specific antibodies, and NaDodSO4/polyacrylamide gel electrophoresis, that this glycolipid is attached to the variant surface glycoprotein polypeptide within 1 min after its translation.",

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AU - Hereld, D.

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AU - Hart, G. W.

AU - Englund, P. T.

PY - 1985

Y1 - 1985

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AB - The variant surface glycoprotein of the parasite Trypanosoma brucei contains a glycolipid of unknown structure covalently attached to its COOH terminus. We have shown, by using metabolic labeling with [35S]methionine or [3H]myristic acid, precipitation with specific antibodies, and NaDodSO4/polyacrylamide gel electrophoresis, that this glycolipid is attached to the variant surface glycoprotein polypeptide within 1 min after its translation.

UR - https://www.scopus.com/pages/publications/0021867815

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DO - 10.1073/pnas.82.10.3207

M3 - Article

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AN - SCOPUS:0021867815

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JF - Proceedings of the National Academy of Sciences of the United States of America

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ER -

Rapid processing of the carboxyl terminus of a trypanosome variant surface glycoprotein

Abstract

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