Rapid Frequency-Scanned Fiber-Optic Fluorometer Capable of Subnanosecond Lifetime Determinations

Frank V. Bright; Curtis A. Monnig; Gary M. Hieftje

doi:10.1021/ac00127a049

Rapid Frequency-Scanned Fiber-Optic Fluorometer Capable of Subnanosecond Lifetime Determinations

Frank V. Bright
, Curtis A. Monnig
, Gary M. Hieftje

Chemistry

Indiana University Bloomington

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A new fluorescence lifetime instrument is described that allows the rapid acquisition of the frequency-response spectrum of a fluorescent sample. The instrument employs for excitation a continuous wave (CW) or mode-locked argon-ion laser and is capable of scanning and acquiring in less than 10 ms the complete frequency-domain spectrum between 250 and 1000 MHz. Following a simple data-analysis scheme, the fluorescence lifetime of the analyte can be easily calculated. Fluorescence lifetimes for several common nanosecond and subnanosecond fiuorophores are determined and shown to agree with previously reported values.

Original language	English
Pages (from-to)	3139-3144
Number of pages	6
Journal	Analytical Chemistry
Volume	58
Issue number	14
DOIs	https://doi.org/10.1021/ac00127a049
State	Published - Dec 1986

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title = "Rapid Frequency-Scanned Fiber-Optic Fluorometer Capable of Subnanosecond Lifetime Determinations",

abstract = "A new fluorescence lifetime instrument is described that allows the rapid acquisition of the frequency-response spectrum of a fluorescent sample. The instrument employs for excitation a continuous wave (CW) or mode-locked argon-ion laser and is capable of scanning and acquiring in less than 10 ms the complete frequency-domain spectrum between 250 and 1000 MHz. Following a simple data-analysis scheme, the fluorescence lifetime of the analyte can be easily calculated. Fluorescence lifetimes for several common nanosecond and subnanosecond fiuorophores are determined and shown to agree with previously reported values.",

author = "Bright, \{Frank V.\} and Monnig, \{Curtis A.\} and Hieftje, \{Gary M.\}",

year = "1986",

month = dec,

doi = "10.1021/ac00127a049",

language = "English",

volume = "58",

pages = "3139--3144",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

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T1 - Rapid Frequency-Scanned Fiber-Optic Fluorometer Capable of Subnanosecond Lifetime Determinations

AU - Bright, Frank V.

AU - Monnig, Curtis A.

AU - Hieftje, Gary M.

PY - 1986/12

Y1 - 1986/12

N2 - A new fluorescence lifetime instrument is described that allows the rapid acquisition of the frequency-response spectrum of a fluorescent sample. The instrument employs for excitation a continuous wave (CW) or mode-locked argon-ion laser and is capable of scanning and acquiring in less than 10 ms the complete frequency-domain spectrum between 250 and 1000 MHz. Following a simple data-analysis scheme, the fluorescence lifetime of the analyte can be easily calculated. Fluorescence lifetimes for several common nanosecond and subnanosecond fiuorophores are determined and shown to agree with previously reported values.

AB - A new fluorescence lifetime instrument is described that allows the rapid acquisition of the frequency-response spectrum of a fluorescent sample. The instrument employs for excitation a continuous wave (CW) or mode-locked argon-ion laser and is capable of scanning and acquiring in less than 10 ms the complete frequency-domain spectrum between 250 and 1000 MHz. Following a simple data-analysis scheme, the fluorescence lifetime of the analyte can be easily calculated. Fluorescence lifetimes for several common nanosecond and subnanosecond fiuorophores are determined and shown to agree with previously reported values.

UR - https://www.scopus.com/pages/publications/4243902354

U2 - 10.1021/ac00127a049

DO - 10.1021/ac00127a049

M3 - Article

AN - SCOPUS:4243902354

SN - 0003-2700

VL - 58

SP - 3139

EP - 3144

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 14

ER -

Rapid Frequency-Scanned Fiber-Optic Fluorometer Capable of Subnanosecond Lifetime Determinations

Abstract

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