Rapid Enumeration of S-Phase Cells by Means of Monoclonal Antibodies

Azra Raza; Harvey D. Preisler; George L. Mayers; Richard Bankert

doi:10.1056/NEJM198404123101518

Rapid Enumeration of S-Phase Cells by Means of Monoclonal Antibodies

Azra Raza
, Harvey D. Preisler
, George L. Mayers
, Richard Bankert

Microbiology and Immunology

Roswell Park Cancer Institute

Research output: Contribution to journal › Letter › peer-review

59 Scopus citations

Abstract

To the Editor: We report a rapid technique for estimating the amount of S-phase cells in a sample with use of monoclonal antibodies (RPMB I through VI) directed against bromdeoxyuridine. Cells (1X10⁷ per milliliter) are incubated with 10^-4 M bromdeoxyuridine in the presence of 10^-5 M fluorodeoxyuridine for 30 minutes, so that any cell in S-phase will incorporate bromdeoxyuridine in place of thymidine in its DNA. Three drops of this suspension are gently pipetted on coverslips coated with Alcian blue. The adherent cells are treated with 70 per cent ethanol to allow materials to permeate the cell membranes. After hydrochloric.

Original language	English
Pages (from-to)	991
Number of pages	1
Journal	New England Journal of Medicine
Volume	310
Issue number	15
DOIs	https://doi.org/10.1056/NEJM198404123101518
State	Published - Apr 12 1984

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title = "Rapid Enumeration of S-Phase Cells by Means of Monoclonal Antibodies",

abstract = "To the Editor: We report a rapid technique for estimating the amount of S-phase cells in a sample with use of monoclonal antibodies (RPMB I through VI) directed against bromdeoxyuridine. Cells (1X107 per milliliter) are incubated with 10-4 M bromdeoxyuridine in the presence of 10-5 M fluorodeoxyuridine for 30 minutes, so that any cell in S-phase will incorporate bromdeoxyuridine in place of thymidine in its DNA. Three drops of this suspension are gently pipetted on coverslips coated with Alcian blue. The adherent cells are treated with 70 per cent ethanol to allow materials to permeate the cell membranes. After hydrochloric.",

author = "Azra Raza and Preisler, \{Harvey D.\} and Mayers, \{George L.\} and Richard Bankert",

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T1 - Rapid Enumeration of S-Phase Cells by Means of Monoclonal Antibodies

AU - Raza, Azra

AU - Preisler, Harvey D.

AU - Mayers, George L.

AU - Bankert, Richard

PY - 1984/4/12

Y1 - 1984/4/12

N2 - To the Editor: We report a rapid technique for estimating the amount of S-phase cells in a sample with use of monoclonal antibodies (RPMB I through VI) directed against bromdeoxyuridine. Cells (1X107 per milliliter) are incubated with 10-4 M bromdeoxyuridine in the presence of 10-5 M fluorodeoxyuridine for 30 minutes, so that any cell in S-phase will incorporate bromdeoxyuridine in place of thymidine in its DNA. Three drops of this suspension are gently pipetted on coverslips coated with Alcian blue. The adherent cells are treated with 70 per cent ethanol to allow materials to permeate the cell membranes. After hydrochloric.

AB - To the Editor: We report a rapid technique for estimating the amount of S-phase cells in a sample with use of monoclonal antibodies (RPMB I through VI) directed against bromdeoxyuridine. Cells (1X107 per milliliter) are incubated with 10-4 M bromdeoxyuridine in the presence of 10-5 M fluorodeoxyuridine for 30 minutes, so that any cell in S-phase will incorporate bromdeoxyuridine in place of thymidine in its DNA. Three drops of this suspension are gently pipetted on coverslips coated with Alcian blue. The adherent cells are treated with 70 per cent ethanol to allow materials to permeate the cell membranes. After hydrochloric.

UR - https://www.scopus.com/pages/publications/0021342348

U2 - 10.1056/NEJM198404123101518

DO - 10.1056/NEJM198404123101518

M3 - Letter

C2 - 6366566

AN - SCOPUS:0021342348

SN - 0028-4793

VL - 310

SP - 991

JO - New England Journal of Medicine

JF - New England Journal of Medicine

IS - 15

ER -

Rapid Enumeration of S-Phase Cells by Means of Monoclonal Antibodies

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