Radioimmunoassay measurement of ACTH-facilitated PGE2 and PGF2α release from isolated cat adrenocortical cells

Suzanne G. Laychock; R. P. Rubin

doi:10.1016/0090-6980(76)90075-7

Radioimmunoassay measurement of ACTH-facilitated PGE₂ and PGF_2α release from isolated cat adrenocortical cells

Suzanne G. Laychock
, R. P. Rubin

Pharmacology and Toxicology

Virginia Commonwealth University

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Prostaglandin (PG) biosynthesis by trypsin-dispersed cat adrenocortical cells was studied by radioimmunoassay (RIA). Parallel assays of incubation media using PGF_2α and PGF_1α antisera established that PGF_2α is the primary PGF released by feline cortical cells. Following the reduction of PGE to PGF with sodium borohydride (NaBH₄) these same two antisera were also used to identify PGE₂ as the primary PGE released. RIA using a PGE antiserum confirmed the presence of PGE in the incubation medium. Steroidogenic concentrations of ACTH (50-250μU) enhanced PGE and PGF release, and indomethacin suppressed the ACTH-facilitated release. These studies provide additional evidence for ACTH-induced PG synthesis by feline cortical cells, and support the hypothesis that PGs play some role in the steroidogenic action of ACTH.

Original language	English
Pages (from-to)	753-767
Number of pages	15
Journal	Prostaglandins
Volume	11
Issue number	4
DOIs	https://doi.org/10.1016/0090-6980(76)90075-7
State	Published - Apr 1976

Access to Document

10.1016/0090-6980(76)90075-7

Cite this

@article{e623576d1e364963ab85dbb089ff29ee,

title = "Radioimmunoassay measurement of ACTH-facilitated PGE2 and PGF2α release from isolated cat adrenocortical cells",

abstract = "Prostaglandin (PG) biosynthesis by trypsin-dispersed cat adrenocortical cells was studied by radioimmunoassay (RIA). Parallel assays of incubation media using PGF2α and PGF1α antisera established that PGF2α is the primary PGF released by feline cortical cells. Following the reduction of PGE to PGF with sodium borohydride (NaBH4) these same two antisera were also used to identify PGE2 as the primary PGE released. RIA using a PGE antiserum confirmed the presence of PGE in the incubation medium. Steroidogenic concentrations of ACTH (50-250μU) enhanced PGE and PGF release, and indomethacin suppressed the ACTH-facilitated release. These studies provide additional evidence for ACTH-induced PG synthesis by feline cortical cells, and support the hypothesis that PGs play some role in the steroidogenic action of ACTH.",

author = "Laychock, \{Suzanne G.\} and Rubin, \{R. P.\}",

year = "1976",

month = apr,

doi = "10.1016/0090-6980(76)90075-7",

language = "English",

volume = "11",

pages = "753--767",

journal = "Prostaglandins",

issn = "0090-6980",

publisher = "Elsevier B.V.",

number = "4",

}

TY - JOUR

T1 - Radioimmunoassay measurement of ACTH-facilitated PGE2 and PGF2α release from isolated cat adrenocortical cells

AU - Laychock, Suzanne G.

AU - Rubin, R. P.

PY - 1976/4

Y1 - 1976/4

N2 - Prostaglandin (PG) biosynthesis by trypsin-dispersed cat adrenocortical cells was studied by radioimmunoassay (RIA). Parallel assays of incubation media using PGF2α and PGF1α antisera established that PGF2α is the primary PGF released by feline cortical cells. Following the reduction of PGE to PGF with sodium borohydride (NaBH4) these same two antisera were also used to identify PGE2 as the primary PGE released. RIA using a PGE antiserum confirmed the presence of PGE in the incubation medium. Steroidogenic concentrations of ACTH (50-250μU) enhanced PGE and PGF release, and indomethacin suppressed the ACTH-facilitated release. These studies provide additional evidence for ACTH-induced PG synthesis by feline cortical cells, and support the hypothesis that PGs play some role in the steroidogenic action of ACTH.

AB - Prostaglandin (PG) biosynthesis by trypsin-dispersed cat adrenocortical cells was studied by radioimmunoassay (RIA). Parallel assays of incubation media using PGF2α and PGF1α antisera established that PGF2α is the primary PGF released by feline cortical cells. Following the reduction of PGE to PGF with sodium borohydride (NaBH4) these same two antisera were also used to identify PGE2 as the primary PGE released. RIA using a PGE antiserum confirmed the presence of PGE in the incubation medium. Steroidogenic concentrations of ACTH (50-250μU) enhanced PGE and PGF release, and indomethacin suppressed the ACTH-facilitated release. These studies provide additional evidence for ACTH-induced PG synthesis by feline cortical cells, and support the hypothesis that PGs play some role in the steroidogenic action of ACTH.

UR - https://www.scopus.com/pages/publications/0017080414

U2 - 10.1016/0090-6980(76)90075-7

DO - 10.1016/0090-6980(76)90075-7

M3 - Article

C2 - 183238

AN - SCOPUS:0017080414

SN - 0090-6980

VL - 11

SP - 753

EP - 767

JO - Prostaglandins

JF - Prostaglandins

IS - 4

ER -

Radioimmunoassay measurement of ACTH-facilitated PGE₂ and PGF_2α release from isolated cat adrenocortical cells

Abstract

Access to Document

Other files and links

Fingerprint

Cite this