Radioenzymatic assay for plasma dihydroxyphenylglycol (DHPG), dihydroxymandelic acid (DOMA) and dihydroxyphenylacetic acid (DOPAC)

Joseph L. Izzo; Doreen Greulich

doi:10.1016/0024-3205(83)90798-1

Radioenzymatic assay for plasma dihydroxyphenylglycol (DHPG), dihydroxymandelic acid (DOMA) and dihydroxyphenylacetic acid (DOPAC)

Joseph L. Izzo
, Doreen Greulich

Medicine

University of Rochester

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

An adaptation of the single-isotope radioenzymatic catecholamine assay technique allows simultaneous quantitation of free dihydroxyphenylglycol (DHPG), dihydroxymandelic acid (DOMA), and dihydroxyphenylacetic acid (DOPAC) in small volumes of plasma. Incubation of sample with catechol-O-methyltransferase and S-adenosylmethionine is followed by acidic extractions of metabolites and thin-layer chromatography. O-methylated products of the beta-hydroxylated metabolites DHPG and DOMA are further subjected to periodate cleavage to improve sensitivity. Quantitation by liquid scintillation counting with internal standardization results in a sensitivity of approximately 8, 30 and 70 pg for DHPG, DOMA and DOPAC. Normal values for these three metabolites in resting humans are (mean ± S.D., pg/ml): 1010 ± 280 for DHPG, 2090 ± 720 for DOMA and 3270 ± 1470 for DOPAC.

Original language	English
Pages (from-to)	483-488
Number of pages	6
Journal	Life Sciences
Volume	33
Issue number	5
DOIs	https://doi.org/10.1016/0024-3205(83)90798-1
State	Published - Aug 1 1983

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title = "Radioenzymatic assay for plasma dihydroxyphenylglycol (DHPG), dihydroxymandelic acid (DOMA) and dihydroxyphenylacetic acid (DOPAC)",

abstract = "An adaptation of the single-isotope radioenzymatic catecholamine assay technique allows simultaneous quantitation of free dihydroxyphenylglycol (DHPG), dihydroxymandelic acid (DOMA), and dihydroxyphenylacetic acid (DOPAC) in small volumes of plasma. Incubation of sample with catechol-O-methyltransferase and S-adenosylmethionine is followed by acidic extractions of metabolites and thin-layer chromatography. O-methylated products of the beta-hydroxylated metabolites DHPG and DOMA are further subjected to periodate cleavage to improve sensitivity. Quantitation by liquid scintillation counting with internal standardization results in a sensitivity of approximately 8, 30 and 70 pg for DHPG, DOMA and DOPAC. Normal values for these three metabolites in resting humans are (mean ± S.D., pg/ml): 1010 ± 280 for DHPG, 2090 ± 720 for DOMA and 3270 ± 1470 for DOPAC.",

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doi = "10.1016/0024-3205(83)90798-1",

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T1 - Radioenzymatic assay for plasma dihydroxyphenylglycol (DHPG), dihydroxymandelic acid (DOMA) and dihydroxyphenylacetic acid (DOPAC)

AU - Izzo, Joseph L.

AU - Greulich, Doreen

PY - 1983/8/1

Y1 - 1983/8/1

N2 - An adaptation of the single-isotope radioenzymatic catecholamine assay technique allows simultaneous quantitation of free dihydroxyphenylglycol (DHPG), dihydroxymandelic acid (DOMA), and dihydroxyphenylacetic acid (DOPAC) in small volumes of plasma. Incubation of sample with catechol-O-methyltransferase and S-adenosylmethionine is followed by acidic extractions of metabolites and thin-layer chromatography. O-methylated products of the beta-hydroxylated metabolites DHPG and DOMA are further subjected to periodate cleavage to improve sensitivity. Quantitation by liquid scintillation counting with internal standardization results in a sensitivity of approximately 8, 30 and 70 pg for DHPG, DOMA and DOPAC. Normal values for these three metabolites in resting humans are (mean ± S.D., pg/ml): 1010 ± 280 for DHPG, 2090 ± 720 for DOMA and 3270 ± 1470 for DOPAC.

AB - An adaptation of the single-isotope radioenzymatic catecholamine assay technique allows simultaneous quantitation of free dihydroxyphenylglycol (DHPG), dihydroxymandelic acid (DOMA), and dihydroxyphenylacetic acid (DOPAC) in small volumes of plasma. Incubation of sample with catechol-O-methyltransferase and S-adenosylmethionine is followed by acidic extractions of metabolites and thin-layer chromatography. O-methylated products of the beta-hydroxylated metabolites DHPG and DOMA are further subjected to periodate cleavage to improve sensitivity. Quantitation by liquid scintillation counting with internal standardization results in a sensitivity of approximately 8, 30 and 70 pg for DHPG, DOMA and DOPAC. Normal values for these three metabolites in resting humans are (mean ± S.D., pg/ml): 1010 ± 280 for DHPG, 2090 ± 720 for DOMA and 3270 ± 1470 for DOPAC.

UR - https://www.scopus.com/pages/publications/0020532404

U2 - 10.1016/0024-3205(83)90798-1

DO - 10.1016/0024-3205(83)90798-1

M3 - Article

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AN - SCOPUS:0020532404

SN - 0024-3205

VL - 33

SP - 483

EP - 488

JO - Life Sciences

JF - Life Sciences

IS - 5

ER -

Radioenzymatic assay for plasma dihydroxyphenylglycol (DHPG), dihydroxymandelic acid (DOMA) and dihydroxyphenylacetic acid (DOPAC)

Abstract

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